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Archives of Virology

, Volume 163, Issue 10, pp 2719–2725 | Cite as

Inhibition of expression of BmNPV cg30 by bmo-miRNA-390 is a host response to baculovirus invasion

  • Lequn Kang
  • Minglei Wang
  • Xueliang Cao
  • Shunming Tang
  • Dingguo Xia
  • Xingjia Shen
  • Qiaoling Zhao
Original Article

Abstract

Bombyx mori larvae exhibit in vivo defensive reactions immediately after invasion by a virus. One of these defense systems is to express appropriate microRNAs (miRNAs) to respond to the infection. A novel Bombyx mori–encoded miRNA, bmo-miR-390, was identified previously by high-throughput sequencing. Based on bioinformatic predictions, the Bombyx mori nuclear polyhedrosis virus cg30 gene (BmNPV-cg30) is one of the target genes of bmo-miR-390. In this study, expression vectors with an enhanced green fluorescence protein (EGFP) or a luciferase (luc) reporter gene together with bm-miR-390 or the cg30 3’ UTR were constructed and used to co-transfect BmN cells. Using a dual luciferase reporter (DLR) assay, we found that bmo-miR-390 significantly downregulates the expression of BmNPV-cg30 (P < 0.05) in vitro. Moreover, artificially synthesized bmo-miR-390 mimics enhanced the regulatory effect of bmo-miR-390, while an inhibitor eliminated the inhibitory effect. These results show for the first time that bmo-miR-390 can effectively downregulate the expression of BmNPV-cg30 in BmNPV-infected BmN cells.

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Funding

This work was supported by the National Natural Science Foundation of China (Grant nos. 31372378 and 31372377).

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Lequn Kang
    • 1
  • Minglei Wang
    • 1
  • Xueliang Cao
    • 1
  • Shunming Tang
    • 1
  • Dingguo Xia
    • 1
  • Xingjia Shen
    • 1
  • Qiaoling Zhao
    • 1
  1. 1.Jiangsu University of Science and TechnologyZhenjiangChina

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