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Identification and molecular characterization of a novel circular single-stranded DNA virus associated with yerba mate in Argentina

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Abstract

A single-stranded DNA (ssDNA) virus was detected in Yerba mate samples showing chlorotic linear patterns, chlorotic rings and vein yellowing. The full-genome sequences of six different isolates of this ssDNA circular virus were obtained, which share > 99% sequence identity with each other. The newly identified virus has been tentatively named as yerba mate-associated circular DNA virus (YMaCV). The 2707 nt-long viral genome has two and three open reading frame on its complementary and virion-sense strands, respectively. The coat protein is more similar to that of mastreviruses (44% identity), whereas the replication-associated protein of YMaCV is more similar (49% identity) to that encoded by a recently described, unclassified ssDNA virus isolated on trees in Brazil. This is the first report of a circular DNA virus associated with yerba mate. Its unique genome organization and phylogenetic relationships indicates that YMaCV represents a distinct evolutionary lineage within the ssDNA viruses and therefore this virus should be classified as a member of a new species within an unassigned genus or family.

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Acknowledgements

This work was supported by INTA project PNIND PE 1108072 and FONCYT grant (PICT 2014-1212).

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Authors and Affiliations

  1. Instituto de Patología Vegetal, Centro de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria (IPAVE-CIAP-INTA), Camino 60 Cuadras Km 5,5 (X5020ICA), Córdoba, Argentina

    Nicolás Bejerman, Soledad de Breuil & Claudia Nome

  2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2290 (1425 FBQ) CABA, Buenos Aires, Argentina

    Nicolás Bejerman & Soledad de Breuil

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  1. Nicolás Bejerman
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  2. Soledad de Breuil
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  3. Claudia Nome
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Correspondence to Nicolás Bejerman.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: Elvira Fiallo-Olivé.

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Bejerman, N., de Breuil, S. & Nome, C. Identification and molecular characterization of a novel circular single-stranded DNA virus associated with yerba mate in Argentina. Arch Virol 163, 2811–2815 (2018). https://doi.org/10.1007/s00705-018-3910-y

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