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Development of a TaqMan MGB RT-PCR assay for the detection of type A and subtype H10 avian influenza viruses

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Abstract

H10 subtype avian influenza viruses have caused several epidemics in poultry and mammals, and specific, rapid and sensitive methods for detection are urgently needed. Herein, TaqMan minor groove binder (MGB) probes and multiplex real-time RT-PCR primers were designed based on gene regions encoding conserved domains of the nucleoprotein and H10 hemagglutinin. The developed multiplex real-time RT-PCR assay displayed high specificity, repeatability, and a detection limit of 10 copies per reaction. This diagnostic method could prove valuable for the rapid detection of H10 subtype AIVs in China.

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References

  1. Englund L, Segerstad CH (1998) Two avian H10 influenza A virus strains with different pathogenicity for mink (Mustela vison). Arch Virol 143(4):653–666

    Article  PubMed  CAS  Google Scholar 

  2. Ma C, Lam TTY, Chai YJ, Wang J, Fan XH, Hong WS, Zhang Y, Li LF, Liu YM, Smith DK, Webby RJ, Peiris JSM, Zhu HC, Guan Y (2015) Emergence and evolution of H10 subtype Influenza viruses in poultry in China. J Virol 89(7):3534–3541

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  3. Su C, Chen S, Liu X, Xu J, Huang K, Liu K, Huang J, Peng D, Liu X (2013) Genome sequence of a Novel H10N9 Avian Influenza Virus Isolated from Chickens in a live poultry market in Eastern China. Genome Announc 1(4):e00386-13

    Article  PubMed  PubMed Central  Google Scholar 

  4. Zhang MM, Zhang XX, Xu KD, Teng QY, Liu QF, Li XS, Yang JM, Xu JQ, Chen HJ, Zhang XY, Li ZJ (2017) Characterization of the pathogenesis of H10N3, H10N7, and H10N8 subtype Avian Influenza viruses circulating in ducks. Sci Rep 6:34489

    Article  CAS  Google Scholar 

  5. Jia YN, Yang JY, Wang ZX, Du YY, Cui J, Wang L, Guo FF, Yang MJ, Han SF, Zhu QY (2017) Genetic properties and pathogenicity of a novel reassortant H10N5 influenza virus from wild birds. Arch Virol 162(5):1349–1353

    Article  PubMed  CAS  Google Scholar 

  6. Englund L (2000) Studies on influenza viruses H10N4 and H10N7 of avian origin in mink. Vet Microbiol 74(1–2):101–107

    Article  PubMed  CAS  Google Scholar 

  7. Wang N, Zou W, Yang Y, Guo XB, Hua YF, Zhang Q, Zhao ZZ, Jin ML (2012) Complete genome sequence of an H10N5 Avian Influenza virus isolated from Pigs in Central China. J Virol 86(24):13865–13866

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  8. van den Brand JMA, Wohlsein P, Herfst S, Bodewes R, Pfankuche VM, van de Bildt MWG, Seehusen F, Puff C, Richard M, Siebert U, Lehnert K, Bestebroer T, Lexmond P, Fouchier RAM, Prenger-Berninghoff E, Herbst W, Koopmans M, Osterhaus ADME, Kuiken T, Baumgartner W (2016) Influenza A (H10N7) virus causes respiratory tract disease in Harbor Seals and Ferrets. PLoS One 11(7):e0159625

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  9. Arzey GG, Kirkland PD, Arzey KE, Frost M, Maywood P, Conaty S, Hurt AC, Deng YM, Iannello P, Barr I, Dwyer DE, Ratnamohan M, McPhie K, Selleck P (2012) Influenza Virus A (H10N7) in Chickens and Poultry Abattoir Workers, Australia. Emerg Infect Dis 18(5):814–816

    Article  PubMed  PubMed Central  Google Scholar 

  10. Chen H, Yuan H, Gao R, Zhang J, Wang D, Xiong Y, Fan G, Yang F, Li X, Zhou J, Zou S, Yang L, Chen T, Dong L, Bo H, Zhao X, Zhang Y, Lan Y, Bai T, Dong J, Li Q, Wang S, Zhang Y, Li H, Gong T, Shi Y, Ni X, Li J, Zhou J, Fan J, Wu J, Zhou X, Hu M, Wan J, Yang W, Li D, Wu G, Feng Z, Gao GF, Wang Y, Jin Q, Liu M, Shu Y (2014) Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection: a descriptive study. Lancet 383(9918):714–721

    Article  PubMed  Google Scholar 

  11. Sutton TC, Lamirande EW, Czako R, Subbarao K (2017) Evaluation of the biological properties and cross-reactive antibody response to H10 Influenza viruses in Ferrets. J Virol 91(19):e00895-17

    Article  PubMed Central  Google Scholar 

  12. Wu HB, Peng XM, Peng XR, Cheng LF, Jin CZ, Lu XY, Xie TS, Yao HP, Wu NP (2016) Multiple amino acid substitutions involved in the adaptation of avian-origin influenza A (H10N7) virus in mice. Arch Virol 161(4):977–980

    Article  PubMed  CAS  Google Scholar 

  13. Vemula SV, Zhao JQ, Liu JK, Wang X, Biswas S, Hewlett I (2016) Current approaches for diagnosis of influenza virus infections in humans. Viruses-Basel 8(4):96

    Article  CAS  Google Scholar 

  14. Wu HB, Lu RF, Peng XM, Liu FM, Cheng LF, Wu NP (2017) Characterization of reassortant H1-subtype avian influenza viruses isolated from poultry in Zhejiang Province in China from 2013 to 2015. Arch Virol 162(11):3493–3500

    Article  PubMed  CAS  Google Scholar 

  15. Wu HB, Lu RF, Peng XM, Peng XR, Cheng LF, Liu FM, Wu NP (2017) Characterization of novel reassortant Influenza A (H5N2) viruses isolated from poultry in Eastern China, 2015. Front Microbiol 8:741

    Article  PubMed  PubMed Central  Google Scholar 

  16. Wu HB, Peng XM, Peng XR, Cheng LF, Lu XY, Jin CZ, Xie TS, Yao HP, Wu NP (2015) Genetic and molecular characterization of H9N2 and H5 avian influenza viruses from live poultry markets in Zhejiang Province, eastern China. Sci Rep 5:17508

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  17. Wu HB, Guo CT, Lu RF, Xu LH, Wo EK, You JB, Wang YT, Wang QG, Wu NP (2012) Characterization of a highly pathogenic H5N1 avian influenza virus isolated from ducks in Eastern China in 2011. Arch Virol 157(6):1131–1136

    Article  CAS  Google Scholar 

  18. Yang F, Wu H, Liu F, Lu X, Peng X, Wu N (2018) Establishment of a multiplex real-time RT-PCR assay for rapid identification of H6 subtype avian influenza viruses. Arch Virol 163(6):1671–1675

    Article  PubMed  CAS  Google Scholar 

  19. Zhang Y, Mao H, Yan J, Wang X, Zhang L, Guus K, Li H, Li Z, Chen Y, Gong L, Chen Z, Xia S (2014) Development of novel AllGlo-probe-based one-step multiplex qRT-PCR assay for rapid identification of avian influenza virus H7N9. Arch Virol 159(7):1707–1713

    Article  PubMed  CAS  Google Scholar 

  20. Sidoti F, Rizzo F, Costa C, Astegiano S, Curtoni A, Mandola ML, Cavallo R, Bergallo M (2010) Development of real time RT-PCR assays for detection of type A influenza virus and for subtyping of avian H5 and H7 hemagglutinin subtypes. Mol Biotechnol 44(1):41–50

    Article  PubMed  CAS  Google Scholar 

  21. Zhang Z, Liu D, Sun W, Liu J, He L, Hu J, Gu M, Wang X, Liu X, Hu S, Chen S, Peng D, Liu X (2017) Multiplex one-step real-time PCR by Taqman-MGB method for rapid detection of pan and H5 subtype avian influenza viruses. PLoS One 12(6):e0178634

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  22. Wu H, Lu R, Wu X, Peng X, Xu L, Cheng L, Lu X, Jin C, Xie T, Yao H, Wu N (2015) Isolation and characterization of a novel H10N2 avian influenza virus from a domestic duck in Eastern China. Infect Genet Evol 29:1–5

    Article  PubMed  Google Scholar 

  23. Krog JS, Hansen MS, Holm E, Hjulsager CK, Chriel M, Pedersen K, Andresen LO, Abildstrom M, Jensen TH, Larsen LE (2015) Influenza A(H10N7) virus in dead harbor seals, Denmark. Emerg Infect Dis 21(4):684–687

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  24. Luo SS, Xie ZX, Xie LJ, Liu JB, Xie ZQ, Deng XW, Huang L, Huang JL, Zeng TT, Khan MI (2015) Reverse-transcription, loop-mediated isothermal amplification assay for the sensitive and rapid detection of H10 subtype avian influenza viruses. Virol J 12:145

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  25. Sun H, Xue JL, Bailey E, Xu Y, Hu G, Baroch J, Zhang Y, Pace L, DeLiberto TJ, Wan XF (2016) A quantitative RT-PCR assay for rapid detection of Eurasian-lineage H10 subtype influenza a virus. Virol Sin 31(5):444–447

    Article  PubMed  PubMed Central  Google Scholar 

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Funding

This study was funded by Grants from the National Science Foundation of the People’s Republic of China (81502852) and the Independent Task of State Key Laboratory for Diagnosis and Treatment of Infectious Diseases (Nos. 2017ZZ10 and 2018ZZ06).

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Authors and Affiliations

  1. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, China

    Fan Yang, Bin Chen, Fumin Liu, Xiuming Peng, Tao Sun, Hangping Yao, Haibo Wu & Nanping Wu

Authors
  1. Fan Yang
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  2. Bin Chen
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  3. Fumin Liu
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  4. Xiuming Peng
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  5. Tao Sun
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  6. Hangping Yao
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  7. Haibo Wu
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  8. Nanping Wu
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Corresponding author

Correspondence to Haibo Wu.

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The authors declare that they have no conflict of interest.

Additional information

Handling Editor: William G. Dundon.

Electronic supplementary material

Below is the link to the electronic supplementary material.

705_2018_3889_MOESM1_ESM.pdf

Primers and probes used in the multiplex real-time RT-PCR assays. Regions targeted by primer pairs and probes for NA (A) and H10 (B) sequences are boxed. (PDF 1094 kb)

Supplementary material 2 (DOCX 23 kb)

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Yang, F., Chen, B., Liu, F. et al. Development of a TaqMan MGB RT-PCR assay for the detection of type A and subtype H10 avian influenza viruses. Arch Virol 163, 2497–2501 (2018). https://doi.org/10.1007/s00705-018-3889-4

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  • DOI: https://doi.org/10.1007/s00705-018-3889-4

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