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Characterization and application of monoclonal antibodies against channel catfish virus (CCV)

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Abstract

Three monoclonal antibodies (MAbs) (27E4, 17H2, 8B6) against channel catfish virus (CCV) were developed by immunizing Balb/C mice. Using indirect ELISA, these MAbs reacted only with CCV and not with three other fish viruses or nine fish cell lines. During western blotting analysis, MAb 27E4 recognized 170 kDa and 47 kDa proteins, while MAb 17H2 and MAb 8B6 recognized 47 kDa and 56 kDa proteins, respectively. Furthermore, a sandwich ELISA was developed for detection of CCV. The detection limit of the test was 105 TCID50/mL.

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Acknowledgements

This research was supported by the Basic Research Fund for Central Public Interest Institutes of China (2017JK039).

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Authors and Affiliations

  1. Chinese Academy of Inspection and Quarantine, Beijing, 100176, China

    Hongli Jing, Min Zhang, Shaoqiang Wu, Xiangmei Lin & Na Wang

  2. Shekou in Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518067, China

    Longying Gao

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  1. Hongli Jing
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  2. Min Zhang
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  3. Shaoqiang Wu
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  4. Xiangmei Lin
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  5. Longying Gao
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Correspondence to Shaoqiang Wu.

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Handling Editor: Chan-Shing Lin.

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Jing, H., Zhang, M., Wu, S. et al. Characterization and application of monoclonal antibodies against channel catfish virus (CCV). Arch Virol 163, 2503–2506 (2018). https://doi.org/10.1007/s00705-018-3884-9

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  • DOI: https://doi.org/10.1007/s00705-018-3884-9

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