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Genome sequence of the novel virulent bacteriophage PMBT14 with lytic activity against Pseudomonas fluorescens DSM 50090R

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Abstract

Psychrotrophic gram-negative Pseudomonas spp. represent a serious problem in the dairy industry as they can cause spoilage of milk and dairy products. Bacteriophages have moved into focus as promising biocontrol agents for such food spoilage bacteria. The virulent Siphoviridae phage PMBT14 was isolated on a mutant variant of P. fluorescens DSM 50090 challenged with an unrelated virulent P. fluorescens DSM 50090 Podoviridae phage (i.e., mutant strain DSM 50090R). PMBT14 has a 47,820-bp dsDNA genome with 76 predicted open reading frames (ORFs). Its genome shows no significant sequence similarity to that of known phages, suggesting that PMBT14 represents a novel phage. Phage PMBT14 could be a promising biocontrol agent for P. fluorescens in milk or dairy foods.

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Acknowledgements

We kindly thank Angela Back and Gesa Gehrke for technical assistance.

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Authors and Affiliations

  1. Department of Microbiology and Biotechnology, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Hermann-Weigmann-Str. 1, 24103, Kiel, Germany

    Sabrina Koberg, Stefanie Gieschler, Erik Brinks, Horst Neve & Charles M. A. P. Franz

  2. Lehrstuhl für Mikrobielle Ökologie, ZIEL-Institute for Food and Health, Technische Universität München, Weihenstephaner Berg 3, 85354, Freising, Germany

    Mareike Wenning

Authors
  1. Sabrina Koberg
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  2. Stefanie Gieschler
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  3. Erik Brinks
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  4. Mareike Wenning
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  5. Horst Neve
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  6. Charles M. A. P. Franz
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Corresponding author

Correspondence to Sabrina Koberg.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: Johannes Wittmann.

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Koberg, S., Gieschler, S., Brinks, E. et al. Genome sequence of the novel virulent bacteriophage PMBT14 with lytic activity against Pseudomonas fluorescens DSM 50090R. Arch Virol 163, 2575–2577 (2018). https://doi.org/10.1007/s00705-018-3882-y

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  • DOI: https://doi.org/10.1007/s00705-018-3882-y