Abstract
Bacteriophage Deep-Purple, isolated from an agricultural soil in Belgium, lyses the emetic Bacillus weihenstephanensis strain LH002 and exhibits a lytic activity against 55% of emetic Bacillus cereus and B. weihenstephanensis strains. Deep-Purple is able to complete its lytic cycle within 45 min and is stable to a large range of pHs and temperatures below 60 °C. It possesses an icosahedral head of about 63 nm in diameter and a non-contractile tail of approximately 165 nm in length. The genome of this newly classifiable Siphoviridae family member is 36,278 bp long, with a G+C content of 38.36% and 40 putative CDSs. Most CDSs do not display similarity with other B. cereus group phages supporting the idea that Deep-Purple belongs to a new and currently uncharacterised Siphoviridae subfamily.
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Acknowledgements
We are grateful to P. Lipnik and D. Magnin for their technical assistance with transmission electron microscopy.
Funding
This study was funded by the FRIA (Fonds pour la formation à la Recherche dans l’Industrie et dans l’Agriculture) through a grant to L. Hock, the FNRS (National Fund for Scientific Research) through grants to A. Gillis and J. Mahillon, the Université catholique de Louvain and the Research Department of the Communauté française de Belgique (Concerted Research Action, ARC12_17-046).
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L. Hock, A. Gillis and J. Mahillon declare that they have no conflict of interest.
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Hock, L., Gillis, A. & Mahillon, J. Complete genome sequence of bacteriophage Deep-Purple, a novel member of the family Siphoviridae infecting Bacillus cereus. Arch Virol 163, 2555–2559 (2018). https://doi.org/10.1007/s00705-018-3865-z
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DOI: https://doi.org/10.1007/s00705-018-3865-z