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High-throughput sequencing reveals a novel closterovirus in arracacha (Arracacia xanthorrhiza)

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Abstract

High-throughput sequencing analysis detected a clostero-like virus from arracacha plants (Arracacia xanthorrhiza) in Brazil. The complete genome sequence, confirmed by RACE and Sanger sequencing, consists of 15,763 nucleotides with nine predicted open reading frames (ORFs) in a typical closterovirus genome organisation. The putative RNA-dependent RNA polymerase (RdRp), heat shock protein 70 homologue (Hsp70h), and coat protein showed 55-65, 38-44, and 20-36% amino acid sequence identity, respectively, to the homologous proteins of known closteroviruses. Phylogenetic analysis of Hsp70h showed that this putative novel arracacha plant virus was related to members of the genus Closterovirus in the family Closteroviridae. These results suggest that this virus, tentatively named “arracacha virus 1” (AV-1), is a novel member of the genus Closterovirus. This is the first closterovirus identified in arracacha plants.

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Acknowledgements

This work was supported by CNPq and EMBRAPA. A. F. O. was a recipient of a fellowship obtained from the CNPq-CsF Program. G. A. C. obtained a graduate fellowship from the PIBIC-UnB Program. TN, AKIN and ROR are CNPq fellows.

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Authors and Affiliations

  1. Department of Cell Biology, University of Brasilia, Campus Universitário Darcy Ribeiro, Brasília, Distrito Federal, 70910-900, Brazil

    Anelise F. Orílio, Rosana Blawid, Gabriela A. Costa, Suzane S. V. S. F. Gomes, Tatsuya Nagata & Renato O. Resende

  2. Embrapa Vegetables, Caixa Postal 218, Brasília, DF, 70275-970, Brazil

    Nuno R. Madeira & Alice K. Inoue-Nagata

Authors
  1. Anelise F. Orílio
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  2. Rosana Blawid
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  3. Gabriela A. Costa
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  4. Suzane S. V. S. F. Gomes
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  5. Tatsuya Nagata
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  6. Nuno R. Madeira
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  7. Alice K. Inoue-Nagata
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  8. Renato O. Resende
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Correspondence to Anelise F. Orílio.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: Massimo Turina.

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Orílio, A.F., Blawid, R., Costa, G.A. et al. High-throughput sequencing reveals a novel closterovirus in arracacha (Arracacia xanthorrhiza). Arch Virol 163, 2547–2550 (2018). https://doi.org/10.1007/s00705-018-3863-1

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  • DOI: https://doi.org/10.1007/s00705-018-3863-1

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