Development and evaluation of a rapid recombinase polymerase amplification assay for the detection of human enterovirus 71

  • Dan Yin
  • Yanan Zhu
  • Kaifeng Wang
  • Jing Wang
  • Xiru Zhang
  • Min Han
  • Yaqing He
  • Qing Chen
  • Guifang Hu
Brief Report
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Abstract

Enterovirus 71 (EV71) is one of the most common pathogens of hand, foot, and mouth disease (HFMD). A rapid reverse transcription recombinase polymerase amplification (RT-RPA) assay was established to detect EV71 subgenotype C4 (EV71-C4). The 95% detection limit of the RT-RPA was 3.767 log10 genomic copies (LGC)/reaction. The specificity was 100%. In a clinical sample evaluation, this approach demonstrated sufficient clinical performance when compared with a commercial RT-qPCR diagnostic kit. Thus, the RT-RPA assay may be a promising alternative for the detection of EV71-C4.

Notes

Funding

This study was supported by the Guangdong Province Public Welfare Research and Capacity Building Project (Grant number: 2014B020212007) and the Guangzhou Science and Technology Project (Grant number: 201508020018).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Ethical approval

This study was approved by the Ethics Committee of the Nanfang Hospital, Southern Medical University, Guangzhou, China.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Dan Yin
    • 1
  • Yanan Zhu
    • 1
  • Kaifeng Wang
    • 2
  • Jing Wang
    • 1
  • Xiru Zhang
    • 1
  • Min Han
    • 1
  • Yaqing He
    • 3
  • Qing Chen
    • 1
  • Guifang Hu
    • 1
  1. 1.Department of Epidemiology, School of Public HealthSouthern Medical UniversityGuangzhouChina
  2. 2.Department of Infectious Diseases, Nanfang HospitalSouthern Medical UniversityGuangzhouChina
  3. 3.Shenzhen Center for Disease Control and PreventionShenzhenChina

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