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Archives of Virology

, Volume 163, Issue 7, pp 1823–1829 | Cite as

Detection and genetic characterization of porcine astroviruses in piglets with and without diarrhea in Thailand

  • Kattareeya Kumthip
  • Pattara Khamrin
  • Wilaiporn Saikruang
  • Aphisek Kongkaew
  • Ratchaya Vachirachewin
  • Hiroshi Ushijima
  • Niwat Maneekarn
Original Article
  • 211 Downloads

Abstract

Porcine astrovirus (PAstV) is widely distributed and highly prevalent among pigs, nevertheless its clinical significance remains unclear as it can be detected in both diarrheic and in healthy pigs. Information about the prevalence, clinical significance and molecular characterization of PAstV in Thailand is not available. This study investigated the prevalence of PAstV in 488 fecal samples collected from piglets with and without diarrhea in 28 pig farms in northern and central parts of Thailand using RT-PCR. The overall prevalence of PAstV infection was 6.5% (32/488), of which 21/251 (8.4%) were in diarrheic and 11/237 (4.6%) were in healthy pigs. Of 32 positive samples, 46.9% were positive for PAstV alone whereas 53.1% were co-infected with porcine group A rotavirus (PRVA). A phylogenetic analysis of the partial RNA-dependent RNA polymerase/capsid genes revealed two lineages of PAstV strains detected in this study. PAstV4 was the most dominant genotype (92%), followed by PAstV2 (8%). This study revealed for the first time that PAstV4 and PAstV2 were circulating in Thailand with PAstV4 as the most dominant genotype in pig herds in northern and central parts of Thailand.

Notes

Acknowledgements

This work was supported by the Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This study was approved by the Research Ethics Committee of the Faculty of Medicine, Chiang Mai University (MIC-2560-04495).

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Kattareeya Kumthip
    • 1
    • 2
  • Pattara Khamrin
    • 1
    • 2
  • Wilaiporn Saikruang
    • 1
  • Aphisek Kongkaew
    • 2
    • 3
  • Ratchaya Vachirachewin
    • 2
    • 4
  • Hiroshi Ushijima
    • 5
  • Niwat Maneekarn
    • 1
    • 2
  1. 1.Department of Microbiology, Faculty of MedicineChiang Mai UniversityChiang MaiThailand
  2. 2.Center of Excellence in Emerging and Re-emerging Diarrheal VirusesChiang Mai UniversityChiang MaiThailand
  3. 3.Animal House Unit, Faculty of MedicineChiang Mai UniversityChiang MaiThailand
  4. 4.Department of Food Animal Clinic, Faculty of Veterinary MedicineChiang Mai UniversityChiang MaiThailand
  5. 5.Division of Microbiology, Department of Pathology and MicrobiologyNihon University School of MedicineTokyoJapan

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