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Archives of Virology

, Volume 163, Issue 7, pp 1779–1793 | Cite as

Co-circulation of multiple genotypes of influenza A (H7N9) viruses in eastern China, 2016-2017

  • Xian Qi
  • Xiaofei An
  • Yongjun Jiao
  • Huiyan Yu
  • Ke Xu
  • Lunbiao Cui
  • Shenjiao Wang
  • Fei Deng
  • Xiang Huo
  • Haodi Huang
  • Qigang Dai
  • Changjun Bao
Original Article

Abstract

Five epidemic waves of human infection with influenza A (H7N9) virus have emerged in China since spring 2013. We previously described the epidemiological characterization of the fifth wave in Jiangsu province. In this study, 41 H7N9 viruses from patients and live-poultry markets were isolated and sequenced to further elucidate the genetic features of viruses of the fifth wave in Jiangsu province. Phylogenetic analysis revealed substantial genetic diversity in the internal genes, and 18 genotypes were identified from the 41 H7N9 virus strains. Furthermore, our data revealed that 41 isolates from Jiangsu contained the G186V and Q226L/I mutations in their haemagglutinin (HA) protein, which may increase the ability of these viruses to bind the human receptor. Four basic amino acid insertions were not observed in the HA cleavage sites of 167 H7N9 viruses from Jiangsu, which revealed that highly pathogenic avian influenza (HPAI) H7N9 viruses did not spread to Jiangsu province in the fifth wave. These findings revealed that multiple genotypes of H7N9 viruses co-circulated in the fifth wave in Jiangsu province, which indicated that the viruses have undergone ongoing evolution with genetic mutation and reassortment. Our study highlights the need to constantly monitor the evolution of H7N9 viruses and reinforce systematic influenza surveillance of humans, birds, and pigs in China.

Notes

Funding

This study was supported by Jiangsu Provincial Medical Talent Project (No. ZDRCA2016031 and ZDRC A2016032), Science & Technology Demonstration Project for Major Emerging Infectious Diseases Control and Prevention (No. BE2015714, BE2017749), Key Medical Discipline of Epidemiology (No. ZDXK A2016008) and the ‘333’ Project in Jiangsu Province.

Compliance with ethical standards

Conflict of interest

The authors declare that we have no conflicts of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of Jiangsu Provincial Center for Disease Control and Prevention.

Informed consent

Informed consent was obtained from all individual participants included in this study.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Xian Qi
    • 1
  • Xiaofei An
    • 2
  • Yongjun Jiao
    • 1
  • Huiyan Yu
    • 1
  • Ke Xu
    • 1
  • Lunbiao Cui
    • 1
  • Shenjiao Wang
    • 1
  • Fei Deng
    • 1
  • Xiang Huo
    • 1
  • Haodi Huang
    • 1
  • Qigang Dai
    • 1
  • Changjun Bao
    • 1
  1. 1.Jiangsu Provincial Center for Disease Control and PreventionJiangsuChina
  2. 2.Department of Endocrinology, Jiangsu Province Hospital of Chinese MedicineAffiliated Hospital of Nanjing University of Chinese MedicineNanjingChina

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