Complete nucleotide sequence of a new carlavirus in chrysanthemums in China
A new virus causing a serious stunt disease of chrysanthemum was identified in China by high-throughput sequencing (HTS) and named chrysanthemum virus R (CVR). The complete sequence of CVR was determined by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The genomic RNA of CVR consists of 8,874 nucleotides (nt), excluding the poly(A) tail, contains six putative open reading frames (ORFs), and has a genomic organization typical of members of the genus Carlavirus. BLAST analysis of the full genome sequence showed low similarity (38%–56% sequence identity) to other members of the genus Carlavirus. BLAST analysis and phylogenetic analysis based on the amino acid (aa) sequences of the CVR replicase and coat protein (CP) confirmed that CVR is a distinct member of the genus Carlavirus.
Compliance with ethical standards
This work was supported by the Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences (Grant number 2016-I2M-3-017).
Conflict of interest
The authors declare that they have no conflict of interest.
Research involving human participants or animals
This study did not include experiments with human participants or animals performed by any of the authors.
- 1.Adams MJ, Candresse T, Hammond J et al (2012) Betaflexiviridae. In: King AMQ, Adams MJ, Carstens EB et al (eds) Virus taxonomy: ninth report of the international committee on taxonomy of viruses. Elsevier, San Diego, pp 920–941Google Scholar