Archives of Virology

, Volume 163, Issue 7, pp 1973–1976 | Cite as

Complete nucleotide sequence of a new carlavirus in chrysanthemums in China

Annotated Sequence Record

Abstract

A new virus causing a serious stunt disease of chrysanthemum was identified in China by high-throughput sequencing (HTS) and named chrysanthemum virus R (CVR). The complete sequence of CVR was determined by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The genomic RNA of CVR consists of 8,874 nucleotides (nt), excluding the poly(A) tail, contains six putative open reading frames (ORFs), and has a genomic organization typical of members of the genus Carlavirus. BLAST analysis of the full genome sequence showed low similarity (38%–56% sequence identity) to other members of the genus Carlavirus. BLAST analysis and phylogenetic analysis based on the amino acid (aa) sequences of the CVR replicase and coat protein (CP) confirmed that CVR is a distinct member of the genus Carlavirus.

Notes

Compliance with ethical standards

Funding

This work was supported by the Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences (Grant number 2016-I2M-3-017).

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants or animals

This study did not include experiments with human participants or animals performed by any of the authors.

Supplementary material

705_2018_3791_MOESM1_ESM.doc (38 kb)
Supplementary material 1 (DOC 38 kb)
705_2018_3791_MOESM2_ESM.doc (114 kb)
Supplementary material 2 (DOC 114 kb)
705_2018_3791_MOESM3_ESM.txt (9 kb)
Supplementary material 3 (TXT 8 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina
  2. 2.Shandong Agricultural UniversityTai’anChina
  3. 3.State Key Laboratory for Agro-Biotechnology and Department for Plant PathologyChina Agricultural UniversityBeijingChina

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