Recovery of mycobacteriophages from archival stocks stored for approximately 50 years in Japan
- 89 Downloads
Mycobacteriophage archival stocks have been kept for ca. 20–50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreover, the analysis of 12 representative recovered phages led to their classification as belonging to the family Siphoviridae, and seven of them were typed by polymerase chain reaction (PCR) targeting the gene that encodes the tape measure protein. Considering these results, lyophilization seems to be suitable for phage archival storage.
The authors thank the staff of the Department of Microbiology and Infection, Kochi Medical School, Kochi University, Kochi, Japan, for experimental advice. They also thank Mr. Hiroki Tsurui and Mr. Masaya Ogata, Azabu University, Kanagawa, Japan, for their technical help. This research was supported by Research Funding Granted by Kochi University President, and by Research Funding Granted by Azabu University.
Compliance with ethical standards
This study was funded by Research Funding Granted by Kochi University President and Research Funding Granted by Azabu University.
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- 4.Hauduroy P, Rosset W (1963) Attempt at treatment of BCG-vaccinated hamsters with a bacteriophage. Ann Inst Pasteur (Paris) 104:419–420Google Scholar
- 9.Murohashi T, Tokunaga T, Seki M (1959) Isolation of mycobacteriophages and their lytic activities. Med Biol 53:242–246Google Scholar
- 10.Murohashi T, Tokunaga T (1960) Classification of mycobacteria by mycophage. Med Biol 56:210–246Google Scholar
- 11.Tokunaga T, Okamoto S, Murohashi T (1960) Studies on the method of sensitivity test of mycophage against Mycobacterium tuberculosis. Med Biol 54:172–177Google Scholar
- 12.Murohashi T, Tokunaga T, Seki M (1960) Effect of freeze-drying on the preservability of mycophage. Med Biol 54:214–218Google Scholar
- 13.Tokunaga T, Seki M, Matsuya T, Murohashi T (1960) Susceptibility of various type of tubercle bacilli to mycophages. Med Biol 54:223–227Google Scholar
- 14.Tokunaga T, Murohashi T, Seki M (1960) Studies on the classification of mycobacteriophages. Med Biol 55:26–30Google Scholar
- 18.Ackermann HW, Tremblay D, Moineau S (2004) Long-term bacteriophage preservation. WFCC Newsl 38:35–40Google Scholar
- 20.Jacobs-Sera D, Marinelli LJ, Bowman C, Broussard GW, Guerrero Bustamante C, Boyle MM, Petrova ZO, Dedrick RM, Pope WH, Science Education Alliance Phage Hunters Advancing G, Evolutionary Science Sea-Phages P, Modlin \ RL, Hendrix RW, Hatfull GF (2012) On the nature of mycobacteriophage diversity and host preference. Virology 434:187–201CrossRefPubMedPubMedCentralGoogle Scholar
- 22.Pedulla ML, Ford ME, Houtz JM, Karthikeyan T, Wadsworth C, Lewis JA, Jacobs-Sera D, Falbo J, Gross J, Pannunzio NR, Brucker W, Kumar V, Kandasamy J, Keenan L, Bardarov S, Kriakov J, Lawrence JG, Jacobs WR Jr, Hendrix RW, Hatfull GF (2003) Origins of highly mosaic mycobacteriophage genomes. Cell 113:171–182CrossRefPubMedGoogle Scholar