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Archives of Virology

, Volume 163, Issue 7, pp 1915–1919 | Cite as

Recovery of mycobacteriophages from archival stocks stored for approximately 50 years in Japan

  • Takako Ujihara
  • Jumpei Uchiyama
  • Tadahiro Nasukawa
  • Hiroki Ando
  • Hironobu Murakami
  • Naoya Ohara
  • Midori Ogawa
  • Toshio Yamazaki
  • Masanori Daibata
  • Masahiro Sakaguchi
  • Shigenobu Matsuzaki
Brief Report

Abstract

Mycobacteriophage archival stocks have been kept for ca. 20–50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreover, the analysis of 12 representative recovered phages led to their classification as belonging to the family Siphoviridae, and seven of them were typed by polymerase chain reaction (PCR) targeting the gene that encodes the tape measure protein. Considering these results, lyophilization seems to be suitable for phage archival storage.

Notes

Acknowledgements

The authors thank the staff of the Department of Microbiology and Infection, Kochi Medical School, Kochi University, Kochi, Japan, for experimental advice. They also thank Mr. Hiroki Tsurui and Mr. Masaya Ogata, Azabu University, Kanagawa, Japan, for their technical help. This research was supported by Research Funding Granted by Kochi University President, and by Research Funding Granted by Azabu University.

Compliance with ethical standards

Funding

This study was funded by Research Funding Granted by Kochi University President and Research Funding Granted by Azabu University.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2018_3788_MOESM1_ESM.pdf (5.8 mb)
Supplementary material 1 (PDF 5971 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Takako Ujihara
    • 1
    • 2
  • Jumpei Uchiyama
    • 3
  • Tadahiro Nasukawa
    • 3
  • Hiroki Ando
    • 4
  • Hironobu Murakami
    • 3
  • Naoya Ohara
    • 5
    • 6
  • Midori Ogawa
    • 7
  • Toshio Yamazaki
    • 8
  • Masanori Daibata
    • 2
  • Masahiro Sakaguchi
    • 3
  • Shigenobu Matsuzaki
    • 2
  1. 1.Science Research CenterKochi UniversityNankokuJapan
  2. 2.Department of Microbiology and Infection, Kochi Medical SchoolKochi UniversityNankokuJapan
  3. 3.School of Veterinary MedicineAzabu UniversitySagamiharaJapan
  4. 4.Department of Microbiology, Graduate School of MedicineGifu UniversityGifuJapan
  5. 5.Department of Oral Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical SciencesOkayama UniversityOkayamaJapan
  6. 6.The Advanced Research Center for Oral and Craniofacial Sciences, Dental SchoolOkayama UniversityOkayamaJapan
  7. 7.Department of Microbiology, School of MedicineUniversity of Occupational and Environmental HealthKitakyushuJapan
  8. 8.Division of Biosafety Control and ResearchNational Institute of Infectious DiseasesTokyoJapan

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