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Archives of Virology

, Volume 163, Issue 6, pp 1677–1681 | Cite as

Characterization of an Australian isolate of taro bacilliform virus and development of an infectious clone

  • Dawit B. Kidanemariam
  • Amit C. Sukal
  • Kathy Crew
  • Grahame V. H. Jackson
  • Adane D. Abraham
  • James L. Dale
  • Robert M. Harding
  • Anthony P. James
Brief Report

Abstract

The badnavirus taro bacilliform virus (TaBV) has been reported to infect taro (Colocasia esculenta L.) and other edible aroids in several South Pacific island countries, but there are no published reports from Australia. Using PCR and RCA, we identified and characterized an Australian TaBV isolate. A terminally redundant cloned copy of the TaBV genome was generated and shown to be infectious in taro following agro-inoculation. This is the first report of TaBV from Australia and also the first report of an infectious clone for this virus.

Notes

Acknowledgements

The authors are grateful to Dr. Ben Dugdale, Queensland University of Technology, for providing the pOPT-NXT vector for cloning purposes. DK is the recipient of an Australia Awards Scholarship.

Data availability

Sequences described in this paper are available under GenBank accession numbers MG017318-MG017320.

Compliance with ethical standards

Conflict of interest

The authors declare they have no conflict of interest.

Ethical approval

This article does not contain any work conducted on animals or human participants.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Centre for Tropical Crops and BiocommoditiesQueensland University of Technology (QUT)BrisbaneAustralia
  2. 2.National Agricultural Biotechnology Research CenterEthiopian Institute of Agricultural ResearchAddis AbabaEthiopia
  3. 3.Department of Agriculture and FisheriesEco-sciences PrecinctBrisbaneAustralia
  4. 4.Grahame JacksonQueens ParkAustralia
  5. 5.Department of BiotechnologyAddis Ababa Science and Technology UniversityAddis AbabaEthiopia

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