Characterization of an Australian isolate of taro bacilliform virus and development of an infectious clone
The badnavirus taro bacilliform virus (TaBV) has been reported to infect taro (Colocasia esculenta L.) and other edible aroids in several South Pacific island countries, but there are no published reports from Australia. Using PCR and RCA, we identified and characterized an Australian TaBV isolate. A terminally redundant cloned copy of the TaBV genome was generated and shown to be infectious in taro following agro-inoculation. This is the first report of TaBV from Australia and also the first report of an infectious clone for this virus.
The authors are grateful to Dr. Ben Dugdale, Queensland University of Technology, for providing the pOPT-NXT vector for cloning purposes. DK is the recipient of an Australia Awards Scholarship.
Sequences described in this paper are available under GenBank accession numbers MG017318-MG017320.
Compliance with ethical standards
Conflict of interest
The authors declare they have no conflict of interest.
This article does not contain any work conducted on animals or human participants.
- 4.Geering A, Hull R (2012) Caulimoviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy, ninth report of the International Committee on Taxonomy of Viruses. Elsevier, Amsterdam, pp 429–443Google Scholar
- 5.Gollifer D, Jackson G, Dabek A, Plumb R, May Y (1977) The occurrence and transmission of viruses of edible aroids in the Solomon Islands and the Southwest Pacific. Int J Pest Manag 23:171–177Google Scholar
- 16.Ming SFY, Ping GW, Ping LW, Xing WX, Ni H (2013) Molecular identification and specific detection of badnavirus from taro grown in China. Acta Phytopathol Sinica 6:590–595Google Scholar