Genomic characteristics of vB_PpaP_PP74, a T7-like Autographivirinae bacteriophage infecting a potato pathogen of the newly proposed species Pectobacterium parmentieri
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Bacteriophage vB_PpaP_PP74 (PP74) is a novel virulent phage that infects members of the species Pectobacterium parmentieri, a newly established species of soft-rot-causing bacteria in the family Pectobacteriaceae, derived from potato-specific Pectobacterium wasabiae. vB_PpaP_PP74 was identified as a member of the family Podoviridae by transmission electron microscopy. The phage has a 39,790-bp dsDNA genome containing 50 open reading frames (ORFs). Because of the absence of genes encoding toxins or lysogeny factors, PP74 may be considered a candidate phage for pathogen biocontrol applications. The genome layout is similar to genomes of T7-like phages within the subfamily Autographivirinae, and therefore, functions can be attributed to most of ORFs. However, the closest nucleotide sequence homologs of phage PP74 are unclassified Escherichia phages. Based on phylogenetic analysis, vB_PpaP_PP74 is a sensu lato T7-like phage, but it forms a distant subgenus group together with homologous enterobacterial phages.
Nucleotide sequence accession number
The complete genome sequence of phage PP74 have been deposited in the NCBI database under the GenBank accession number KY084243. The draft genome assembly of PB20 strain and related information have been deposited in the NCBI database under the GenBank Accession number PDDJ00000000 (BioProject PRJNA400696).
This study was supported by Russian Science Foundation Grant #16-16-00073.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain studies with human participants or animals performed by any of the authors.
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