Development of a nanogold slot blot inhibition assay for the detection of antibodies against bovine herpesvirus type 1
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Bovine herpesvirus type 1 (BoHV-1) is recognized as an important pathogen causing respiratory, reproductive, and neurological disorders in cattle and is associated with economic losses to animal industry. Accurate diagnostic methods are needed for prevention of disease transmission. While the virus neutralization test is considered the gold standard method, it requires maintenance of the virus and cell cultures, which is time consuming and expensive. Serological techniques such as enzyme-linked immunosorbent assay (ELISA) are widely applied, as these are easy to perform and provide quick results. In the present study, a nanogold slot blot inhibition assay was developed for the serological diagnosis of BoHV-1 and compared with standard ELISA and horseradish peroxidase (HRP) slot blot assays. Of 42 serum samples tested by ELISA, 32 (76.2%) were positive and 10 (23.8%), were negative. The sensitivity and specificity of the nanogold slot blot inhibition assay was similar to that observed for ELISA and HRP slot blot assays, and a strong correlation was observed between the tests. Thus, the nanogold slot blot inhibition assay may serve as an efficient and rapid alternative to ELISA in settings, where plate-reading equipment is lacking.
We thank Prof. Dr. Frédérick Jean Georges Frézard (Federal University of Minas Gerais) and Dr. Sydnei Magno da Silva (Federal University of Uberlandia) for the gold nanoparticle analysis.
This study was funded by Conselho Nacional de Desenvolvimento Científico e Tecnológico (Grant number 564204/2010-2) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Grant number 1378410).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with animals performed by any of the authors.
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