A new virus, classifiable in the family Tombusviridae, found infecting Solanum tuberosum in the UK
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A novel virus was discovered in a freeze-dried collection held at SASA, UK, originating from potato (Solanum tuberosum) cv. Nadine. The complete sequence of the viral RNA was determined to be 3674 nucleotides in length encoding five predicted proteins. Based on the deduced genome organization and phylogenetic analysis, this virus represents a putative new member of the genus Alphanecrovirus, family Tombusviridae, most closely related to isolates of Olive mild mosaic virus. The virus was easily transmitted to indicator plants with symptoms that were slower to develop and less severe than those of related viruses. To distinguish this virus, the clearest symptom differences occurred with Nicotiana debneyi, Chenopodium amaranticolor and Ch. quinoa. The virus was detected with antisera to the related viruses tobacco necrosis virus A and tobacco necrosis virus D. The close association to the tobacco necrosis viruses would suggest this virus is not a new introduction to potato but in the past has been misidentified as one of these viruses. The virus isolate has been named potato necrosis virus.
The authors wish to thank Robyn DeYoung, Pacific Agri-Food Research Centre, Canada and Annelien Roenhorst, Plant Protection Service, The Netherlands for providing virus isolates for this work and Ross Holmes, Catherine Thomson, Sandra Goodfellow and Carolyn Nisbet, SASA for technical assistance.
Compliance with ethical standards
Conflict of interest
All authors declare they have no conflict of interests.
Research involving human participation and/or animals
This article does not contain any studies with human participants or animals performed by any of the authors.
All authors declare they have informed consent. This study was funded by SASA as part of a programme by the United Kingdom Quarantine Unit, SASA to become an approved source of high health potato material for Australia (http://www.agriculture.gov.au/biosecurity/risk-analysis/plant/review_potato/final-potato).
- 1.Borodynko N, Hasiow-Jaroszewska B, Pospieszny H (2010) Identification and characterization of an Olive latent virus 1 isolate from a new host: Solanum lycopersicum. J Plant Pathol 92(3):789–792Google Scholar
- 2.Boulila M (2011) Positive selection, molecular recombination structure and phylogenetic reconstruction of members of the family Tombusviridae: implication in virus taxonomy. Genet Mol Biol 34(4). https://doi.org/10.1590/S1415-47572011005000046
- 3.Brunt AA, Crabtree K, Dallwitz MJ, Gibbs AJ, Watson L, Zurcher EJ (eds) (1996) Plant viruses online: descriptions and lists from the VIDE database. Version: 20th August 1996. http://biology.anu.edu.au/Groups/MES/vide/Tobacconecrosisvirus. http://pvo.bio-mirror.cn/descr805.htm
- 7.Cardoso JMS, Felix MR, Clara MIE, Oliveira S (2012) First characterization of infectious cDNA clones of Olive mild mosaic virus. Phytopathol Med 51(2):259–265Google Scholar
- 10.Felix MR, Clara MI (1998) Caracteristicas biologicas e bioquimicas da estirpe G1, do Necrovirus Olive Latent 1 isolado de Olea europaea L. Actas da 2a Reuniao Bienal da Sociedade Portuguesa de Fitopatologia, 67Google Scholar
- 13.Felix MR, Cardoso JMS, Oliveira S, Clara MI (2007) Biological and molecular characterization of Olive latent virus 1. Plant Viruses 1(2):170–176. Global Science Books ISSN: 1749-6209 UKGoogle Scholar
- 16.Jeffries CJ (1998) FAO/IPGRI technical guidelines No. 19, p 84. http://cropgenebank.sgrp.cgiar.org/images/file/management/stogs_potato.pdf
- 20.Rochon DA, (2011) ICTV taxonomic proposal 2011.009a-mP.A.v3 divide the genus necrovirus into 2 new genera, alphanecrovirus and betanecrovirus. http://talk.ictvonline.org/ICTV/proposals/2011.009a-mP.A.v3.split_Necrovirus.pdf
- 21.Russo M, Burgyan J, Martelli GP (1994) The molecular biology of Tombusviridae. Adv Virus Res 44:321–428Google Scholar
- 22.Scheets K, White KA, Rubino L, Martelli G, Rochon D’A (2015) ICTV Taxonomic proposal 2015.007a-rP Divide the genus Carmovirus into three new genera: Alphacarmovirus, Betacarmovirus, and Gammacarmovirus. http://talk.ictvonline.org/ICTV/proposals/2015.007a-rP.A.v1.split_Carmovirus.pdf
- 28.Varanda CMR, Machado M, Martel P, Nolasco G, Clara MIE, Felix MR (2014b) Genetic diversity of the coat protein of Olive mild mosaic virus (OMMV) and Tobacco necrosis virus D (TNVD) isolates and its structural implications. PLoS One 9(10). https://doi.org/10.1371/journal.pone.0110941