Archives of Virology

, Volume 163, Issue 6, pp 1407–1417 | Cite as

Immune responses of mature chicken bone-marrow-derived dendritic cells infected with Newcastle disease virus strains with differing pathogenicity

  • Bin Xiang
  • Wenxian Zhu
  • Yaling Li
  • Pei Gao
  • Jianpeng Liang
  • Di Liu
  • Chan Ding
  • Ming Liao
  • Yinfeng Kang
  • Tao Ren
Original Article

Abstract

Infection of chickens with virulent Newcastle disease virus (NDV) is associated with severe pathology and increased morbidity and mortality. The innate immune response contributes to the pathogenicity of NDV. As professional antigen-presenting cells, dendritic cells (DCs) play a unique role in innate immunity. However, the contribution of DCs to NDV infection has not been investigated in chickens. In this study, we selected two representative NDV strains, i.e., the velogenic NDV strain Chicken/Guangdong/GM/2014 (GM) and the lentogenic NDV strain La Sota, to investigate whether NDVs could infect LPS-activated chicken bone-derived marrow DCs (mature chicken BM-DCs). We compared the viral titres and innate immune responses in mature chicken BM-DCs following infection with those strains. Both NDV strains could infect mature chicken BM-DC, but the GM strain showed stronger replication capacity than the La Sota strain in mature chicken BM-DCs. Gene expression profiling showed that MDA5, LGP2, TLR3, TLR7, IFN-α, IFN-β, IFN-γ, IL-1β, IL-6, IL-18, IL-8, CCL5, IL-10, IL-12, MHC-I, and MHC-II levels were altered in mature DCs after infection with NDVs at all evaluated times postinfection. Notably, the GM strain triggered stronger innate immune responses than the La Sota strain in chicken BM-DCs. However, both strains were able to suppress the expression of some cytokines, such as IL-6 and IFN-α, in mature chicken DCs at 24 hpi. These data provide a foundation for further investigation of the role of chicken DCs in NDV infection.

Abbreviations

NDV

Newcastle disease virus

BM-DCs

Bone-marrow-derived dendritic cells

CEFs

Chicken embryo fibroblasts

MHC

Major histocompatibility complex

PRRs

Pattern recognition receptors

TBST

Tris-buffered saline containing 0.05% Tween-20

SPF

Specific-pathogen-free

Notes

Funding

This study was supported by the National Natural Science Foundation of China (no. 31372412, 31702213), the Chinese Special Fund for Agro-Scientific Research in the Public Interest (no. 201303033), the Specialized Research Fund for Doctoral Program of Higher Education of China (no. 20124404110016), the Science and Technology Projects of Guangdong Province (no. 2013B020224002), the Natural Science Foundation Project (no. 2016YFD0501603), and the Poultry Production Technology of Guangdong System (no. 2016LM1115).

Compliance with ethical standards

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Ethical approval

All animal experiments in the present study were approved by the South China Agricultural University Experimental Animal Welfare Ethics Committee (Permit number: 2016-011[20161101]).

Supplementary material

705_2018_3745_MOESM1_ESM.tif (499 kb)
Supplementary material 1 (TIFF 499 kb) Figure S1 The mRNA expression of PRRs in chicken BM-DCs after LPS stimulation

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Veterinary MedicineSouth China Agricultural UniversityGuangzhouPeople’s Republic of China
  2. 2.National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and ControlGuangzhouPeople’s Republic of China
  3. 3.Key Laboratory of Animal Vaccine DevelopmentMinistry of AgricultureGuangzhouPeople’s Republic of China
  4. 4.Key Laboratory of Zoonosis Prevention and Control of Guangdong ProvinceGuangzhouPeople’s Republic of China
  5. 5.College of Animal Science and TechnologyShihezi UniversityShiheziPeople’s Republic of China
  6. 6.Department of Avian Diseases, Shanghai Veterinary Research InstituteChinese Academy of Agricultural SciencesShanghaiPeople’s Republic of China
  7. 7.Department of Experimental Research, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer MedicineSun Yat-sen University Cancer CenterGuangzhouPeople’s Republic of China

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