Archives of Virology

, Volume 163, Issue 5, pp 1375–1378 | Cite as

Molecular characterization of a divergent strain of calla lily chlorotic spot virus infecting celtuce (Lactuca sativa var. augustana) in China

  • Xiaodong Wu
  • Xiaoyun Wu
  • Wenbin Li
  • Xiaofei Cheng
Annotated Sequence Record


Through sequencing and assembly of small RNAs, an orthotospovirus was identified from a celtuce plant (Lactuca sativa var. augustana) showing vein clearing and chlorotic spots in the Zhejiang province of China. The S, M, and L RNAs of this orthotospovirus were determined to be 3146, 4734, and 8934 nt, respectively, and shared 30.4-72.5%, 43.4-80.8%, and 29.84-82.9% nucleotide sequence identities with that of known orthotospoviruses. The full length nucleoprotein (N) of this orthotospovirus shared highest amino acid sequence identity (90.25%) with that of calla lily chlorotic spot virus isolated from calla lily (CCSV-calla) [China: Taiwan: 2001] and tobacco (CCSV-LJ1) [China: Lijiang: 2014]. Phylogenetic analyses showed that this orthotospovirus is phylogenetically associated with CCSV isolates and clustered with CCSV, tomato zonate spot virus (TZSV), and tomato necrotic spot-associated virus (TNSaV) in a separate sub-branch. These results suggest that this orthotospovirus is a divergent isolate of CCSV and was thus named CCSV-Cel [China: Zhejiang: 2017].



The authors would like to thank Dr. Zhongkai Zhang and Dr. Jiahong Dong from the Yunnan Academy of Agricultural Sciences for their valuable suggestions to this manuscript.


This study was financial funded by the National Natural Science Foundation of China (Grant no: 31671998).

Compliance with ethical standards

Conflict of interest

All authors declare that no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2018_3743_MOESM1_ESM.docx (24 kb)
Supplementary material 1 (DOCX 23 kb)
705_2018_3743_MOESM2_ESM.pptx (62 kb)
Supplementary material 2 (PPTX 62 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of AgricultureNortheast Agricultural UniversityHarbinPeople’s Republic of China

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