Abstract
Feline lymphomas are associated with the transduction and activation of cellular proto-oncogenes, such as c-myc, by feline leukemia virus (FeLV). We describe a polymerase chain reaction assay for detection of myc transduction usable in clinical diagnosis. The assay targets c-myc exons 2 and 3, which together result in a FeLV-specific fusion gene following c-myc transduction. When this assay was conducted on FeLV-infected feline tissues submitted for clinical diagnosis of tumors, myc transduction was detected in 14% of T-cell lymphoma/leukemias. This newly established system could become a useful diagnostic tool in veterinary medicine.
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Acknowledgements
We are grateful to Hajime Tsujimoto (The University of Tokyo) for providing feline samples and FT-G cell line. We thank Katie Oakley, PhD, from Edanz Group (www.edanzediting.com/ac) for editing a draft of this manuscript. This study was supported by the Japanese Society for the Promotion of Science, KAKENHI.
Funding
This study was funded by Japan Society for the Promotion of Science KAKENHI (Grant Number 15H04602).
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Animal studies were conducted following the guidelines for the Care and Use of Laboratory Animals of the Ministry of Education, Culture, Sports, Science and Technology, Japan. All experiments were approved by the Genetic Modification Safety Committee of Yamaguchi University.
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Sumi, R., Miyake, A., Endo, T. et al. Polymerase chain reaction-based detection of myc transduction in feline leukemia virus-infected cats. Arch Virol 163, 1073–1077 (2018). https://doi.org/10.1007/s00705-018-3721-1
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DOI: https://doi.org/10.1007/s00705-018-3721-1