Abstract
Mycovirus infection is a universal phenomenon in the major fungus groups. So far, however, mycoviruses have not been described in the phytopathogenic fungus Leptosphaeria biglobosa, the causal agent of phoma stem canker (blackleg). Here, we report the complete genome sequence of a novel mitovirus, Leptosphaeria biglobosa mitovirus 1 (LbMV1), isolated from the strain H3-38 of L. biglobosa. The LbMV1 genome comprises 2568 nucleotides with a low G+C content of 30%. Using the mitochondrial genetic code, the genome of LbMV1 was found to contain a single large open reading frame that encodes a predicted protein of 756 amino acids. Multiple alignment of the predicted protein sequence showed the highest similarity (51% identity) to the putative RNA-dependent RNA polymerase of Ophiostoma mitovirus 4. In addition, phylogenetic analysis grouped LbMV1 within the genus Mitovirus of the family Narnaviridae. Our results suggest that the double-stranded RNA found in strain H3-38 is the replicating genome of the mitovirus LbMV1, its deletion represents the first evidence of a mycovirus infecting L. biglobosa.
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This work was supported by the National Nature Science Foundation of China (31601588), National Key Research and Development Program of China (2017YFD0201100), and the China Agriculture Research System (CARS-13).
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705_2018_4143_MOESM1_ESM.tif
Supplementary material 1 (TIFF 6253 kb) Fig. S1 Predicted secondary structures of the 5’ and 3’ termini of the LbMV1/H3-38 genome. (A) The predicted 5’ stem-loop structure (B) The predicted 3’ stem-loop structure
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Supplementary material 2 (TIFF 10193 kb) Fig. S2 Alignment of the amino acid sequences of the putative RNA-dependent RNA polymerases of Leptosphaeria biglobosa mitovirus 1 and five other mitoviruses. Six conserved motifs are numbered I to VI
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Supplementary material 3 (TIFF 709 kb) Fig. S3 Strain H3-38 was molecularly identified with primers ITS1/ITS4, LmacF/LmacR and LbigF/LmacR. The lanes 1 and 2 represent the DNA amplicons of H3-38 and the negative control (sterile water), respectively. M represents the DNA molecular marker
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Wang, Y., Xu, Z., Cheng, J. et al. Characterization of a novel RNA virus from the phytopathogenic fungus Leptosphaeria biglobosa related to members of the genus Mitovirus. Arch Virol 164, 913–916 (2019). https://doi.org/10.1007/s00705-018-04143-3
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DOI: https://doi.org/10.1007/s00705-018-04143-3