Abstract
A novel negevirus, tentatively named Manglie virus (MaV), was isolated from Culex tritaeniorhynchus from the village of Manglie, Yunnan, China, in August 2011. It was identified by high-throughput sequencing of cell culture supernatants, and the complete genome was sequenced using an Illumina MiSeq sequencer. The complete MaV genome comprised 9,218 nt encoding three hypothetical proteins and had a poly(A) tail. BLASTn analysis showed that the genome had the greatest similarity to Ngewotan virus strain Nepal22, with query coverage of 100% and 79% identity. Genomic and phylogenetic analyses demonstrated that MaV should be considered a novel negevirus.
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Acknowledgements
The authors would like to thank Guangqian Pei, Feiyang Zhao, and Manli Li for their help during this work.
Funding
This research was supported by grants from the National Natural Science Foundation of China (No. 81273138 and No. 81572045) and the Yunnan Health Training Project of High-Level Talents (grant numbers D-201604).
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Wang, Y., Guo, X., Peng, H. et al. Complete genome sequence of a novel negevirus isolated from Culex tritaeniorhynchus in China. Arch Virol 164, 907–911 (2019). https://doi.org/10.1007/s00705-018-04133-5
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DOI: https://doi.org/10.1007/s00705-018-04133-5