Advertisement

Archives of Virology

, Volume 163, Issue 3, pp 799–803 | Cite as

Genome characterization of an Argentinean isolate of alfalfa leaf curl virus

  • Nicolás Bejerman
  • Verónica Trucco
  • Soledad de Breuil
  • Patricia Rodriguez Pardina
  • Sergio Lenardon
  • Fabián Giolitti
Annotated Sequence Record

Abstract

We investigated the molecular characteristics of an Argentinean isolate of alfalfa leaf curl virus (ALCV-Arg), a virus of the genus Capulavirus in the family Geminiviridae that was isolated from alfalfa plants showing dwarfism. The genome was found to be 2,750 nucleotides in length. In pairwise comparisons, this ALCV isolate shared 83.2% to 92.6% sequence identity with European ALCV isolates. Sequence comparisons and phylogenetic analysis showed that this isolate combines features of strains A and B of ALCV. Recombination analysis showed that ALCV-Arg is a recombinant isolate that was generated by intraspecific recombination between ALCV strains A and B. The results of this study not only show that ALCV-Arg is unique because it combines features of strains A and B but also show that ALCV naturally infects this forage crop on the American continent.

Notes

Acknowledgements

This work was partially supported by the PNPV 1135022 project of INTA.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Funding

This work was partially supported by the PNPV 1135022 project of INTA.

Supplementary material

705_2017_3673_MOESM1_ESM.docx (18 kb)
Supplementary material 1 (DOCX 18 kb)
705_2017_3673_MOESM2_ESM.docx (14 kb)
Supplementary material 2 (DOCX 14 kb)
705_2017_3673_MOESM3_ESM.docx (15 kb)
Supplementary material 3 (DOCX 15 kb)
705_2017_3673_MOESM4_ESM.docx (14 kb)
Supplementary material 4 (DOCX 13 kb)

References

  1. 1.
    Abascal F, Zardoya R, Posada D (2005) ProtTest: selection of best-fit models of protein evolution. Bioinformatics 21:2104–2105CrossRefPubMedGoogle Scholar
  2. 2.
    Bejerman N, Nome C, Giolitti F, Kitajima E, de Breuil S, Pérez Fernández J, Basigalup D, Cornacchione M, Lenardon S (2011) First report of a Rhabdovirus infecting alfalfa in Argentina. Plant Dis 95:771CrossRefGoogle Scholar
  3. 3.
    Bejerman N, Giolitti F, Trucco V, de Breuil S, Nome C, Lenardon S, Dietzgen R (2015) Complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruses. Virology 483:275–283CrossRefPubMedGoogle Scholar
  4. 4.
    Bejerman N, Giolitti F, Trucco V, de Breuil S, Dietzgen R, Lenardon S (2016) Complete genome sequence of a new enamovirus from Argentina infecting alfalfa plants showing dwarfism symptoms. Arch Virol 161:2029–2032CrossRefPubMedGoogle Scholar
  5. 5.
    Bernardo P, Golden M, Akram M, Naimuddin Nadarajan N et al (2013) Identification and characterisation of a highly divergent geminivirus: evolutionary and taxonomic implications. Virus Res 177:35–45CrossRefPubMedGoogle Scholar
  6. 6.
    Bernardo P, Muhire B, Francois S, Deshoux M, Hartnady P et al (2016) Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa. Virol 493:142–153CrossRefGoogle Scholar
  7. 7.
    Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15Google Scholar
  8. 8.
    Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis Version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874CrossRefPubMedGoogle Scholar
  10. 10.
    Martin DP, Murrell B, Golden M, Khoosal A, Muhire B (2015) RDP4: detection and analysis of recombination patterns in virus genomes. Virus Evol 1:vev003CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Muhire BM, Varsani A, Martin DP (2014) SDT: a virus classification tool based on pairwise sequence alignment and identity calculation. PLoS One 9:e108277CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Roumagnac P, Granier M, Bernardo P, Deshoux M, Ferdinand R et al (2015) Alfalfa leaf curl virus: an aphid-transmitted geminivirus. J Virol 89:9683–9688CrossRefPubMedPubMedCentralGoogle Scholar
  13. 13.
    Santorum JM, Darriba D, Taboada GL, Posada D (2014) jmodeltest.org: selection of nucleotide substitution models on the cloud. Bioinformatics 30:1310–1311CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Susi H, Laine A-L, Filloux D, Kraberger S, Farkas K et al (2017) Genome sequences of a capulavirus infecting Plantago lanceolata in the Åland archipelago of Finland. Arch Virol 162:2041–2045CrossRefPubMedGoogle Scholar
  15. 15.
    Trucco V, de Breuil S, Bejerman N, Lenardon S, Giolitti F (2014) Complete nucleotide sequence of Alfalfa mosaic virus isolated from alfalfa (Medicago sativa L.) in Argentina. Virus Genes 48:562–565CrossRefPubMedGoogle Scholar
  16. 16.
    Trucco V, de Breuil S, Bejerman N, Lenardon S, Giolitti F (2016) Bean leafroll virus (BLRV) in Argentina: molecular characterization and detection in alfalfa fields. Eur J Plant Pathol 146:207–212CrossRefGoogle Scholar
  17. 17.
    Varsani A, Roumagnac P, Fuchs M, Navas-Castillo J, Moriones E et al (2017) Capulavirus and Grablovirus: two new genera in the family Geminiviridae. Arch Virol 162:1819–1831CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2017

Authors and Affiliations

  • Nicolás Bejerman
    • 1
    • 2
  • Verónica Trucco
    • 1
  • Soledad de Breuil
    • 1
    • 2
  • Patricia Rodriguez Pardina
    • 1
  • Sergio Lenardon
    • 1
    • 3
  • Fabián Giolitti
    • 1
  1. 1.IPAVE-CIAP-INTACórdobaArgentina
  2. 2.Concejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2290 (1425 FBQ) CABABuenos AiresArgentina
  3. 3.Facultad de Agronomía y Veterinaria (FAV)Universidad Nacional de Río Cuarto (UNRC)Río CuartoArgentina

Personalised recommendations