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Archives of Virology

, Volume 163, Issue 3, pp 745–750 | Cite as

Species A rotaviruses isolated from hospitalized patients over 5 years of age in Kolkata, India, in 2012/13

  • Mukti Kant Nayak
  • Papiya De
  • Byomkesh Manna
  • Shanta Dutta
  • Uchhal Kumar Bhadra
  • Mamta Chawla-Sarkar
Brief Report

Abstract

In hospital-based diarrhoeal disease surveillance at Infectious Diseases & Beliaghata-General Hospital (May-2012 to April-2013), Kolkata, India, stool samples were collected from patients < 5 years (n = 830) and > 5 years of age (n = 728) hospitalized with diarrhea. Group-A rotavirus (GARV) was identified by ELISA followed by multiplex RT-PCR. In children < 5 years of age, 53.4% of the samples were positive for GARV. In patients > 5 years to 90 years old, only 6.04% (n = 44) tested positive for GARV. G2P[4] strains (n = 16 [36.36%]) were the most prevalent, followed by G9P[4] strains (n = 13 [29.54%]), while P[4]-(n = 30 [68.18%]) was most prevalent among the P genotypes. The GARV strains G2, G9 and P[4] detected in adults clustered together in the phylogenetic tree with the GARV strains identified in children (< 5 years) during the same period. Rotavirus positivity was high among female patients (75%), suggesting that caregivers (mother/grandmother/older-siblings) may get infected through young children or may act as carriers for transmission.

Keywords

rotavirus adults diarrhea Kolkata India 

Notes

Acknowledgements

Indian Council of Medical Research (ICMR), New Delhi, India, and National Institute of Infectious Diseases (NIID), Tokyo, Japan

Compliance with ethical standards

This study was carried out accordance with the Institutional Ethics Committee guidelines letter no. C-48/2012-T & E.

Funding

None.

Conflict of interest

The author declares that there are no conflicts of interest.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2017

Authors and Affiliations

  • Mukti Kant Nayak
    • 1
    • 3
  • Papiya De
    • 1
  • Byomkesh Manna
    • 1
  • Shanta Dutta
    • 1
  • Uchhal Kumar Bhadra
    • 2
  • Mamta Chawla-Sarkar
    • 1
  1. 1.Division of VirologyNational Institute of Cholera and Enteric DiseasesKolkataIndia
  2. 2.Infectious Diseases and Beliaghata General HospitalKolkataIndia
  3. 3.Department of ZoologyB. B. Autonomous CollegeJajpurIndia

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