Archives of Virology

, Volume 163, Issue 5, pp 1307–1316 | Cite as

Isolation and genomic characterization of a novel avian orthoreovirus strain in Korea, 2014

  • Jin-Yong Noh
  • Dong-Hun Lee
  • Tae-Hyun Lim
  • Ji-ho Lee
  • J. Michael Day
  • Chang-Seon Song
Brief Report

Abstract

In this study, we isolated a novel avian reovirus (ARV) strain, K738/14, from a broiler chicken with viral arthritis in South Korea. Genome sequence comparisons showed relatively low nucleotide identity with previously identified ARV strains. Phylogenetic analyses suggested multiple reassortment events between reovirus strain S1133 and reoviruses of Hungarian, Chinese, and US origin had occurred. In addition, recombination analyses showed evidence of intra-segmental recombination in the M2 and S2 genes. Based on our genetic analyses, multiple reassortment events, intra-segmental recombination, and accumulation of point mutations have possibly contributed to the emergence of this novel genotype of ARV, identified in Korea.

Keywords

Avian reovirus Genetic analysis Reassortment Intra-segmental recombination Point mutation 

Notes

Acknowledgements

We thank Woo-tack Hong, Gyeong-bin Gwon, Kyu-jik Kim for excellent technical assistance.

Compliance with ethical standards

Funding

This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through the Animal Disease Management Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (Grant number: 115099-1).

Conflict of interest

The authors have no conflicts of interest to declare.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2017_3667_MOESM1_ESM.pdf (7 kb)
Supplementary material 1 (PDF 7 kb)
705_2017_3667_MOESM2_ESM.pdf (11 kb)
Supplementary material 2 (PDF 10 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Jin-Yong Noh
    • 1
  • Dong-Hun Lee
    • 2
  • Tae-Hyun Lim
    • 3
  • Ji-ho Lee
    • 1
  • J. Michael Day
    • 2
  • Chang-Seon Song
    • 1
  1. 1.Avian Disease Laboratory, College of Veterinary MedicineKonkuk UniversitySeoulRepublic of Korea
  2. 2.US National Poultry Research Center, US Department of AgricultureAthensUSA
  3. 3.Cherrybro Inc.Cheongju-siRepublic of Korea

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