Isolation and molecular characterization of an H5N1 swine influenza virus in China in 2015
In 2015, an H5N1 influenza virus was isolated from a pig in Zhejiang Province, Eastern China. This strain was characterized by whole-genome sequencing with subsequent phylogenetic analysis. Phylogenetic analysis showed that all segments from this strain belonged to clade 2.3.2 and that it had received its genes from poultry influenza viruses in China. A Glu627Lys mutation associated with pathogenicity was observed in the PB2 protein. This strain was moderately pathogenic in mice and was able to replicate without prior adaptation. These results suggest that active surveillance of swine influenza should be used as an early warning system for influenza outbreaks in mammals.
KeywordsSwine influenza virus H5N1 Reassortment Poultry EASTERN China
Compliance with ethical standards
This study was funded by Grants from the National Science Foundation of the People’s Republic of China (81502852), Zhejiang Provincial Natural Science Foundation of China (LY15H190003), and the Independent Task of State Key Laboratory for Diagnosis and Treatment of Infectious Diseases (Nos. 2016ZZ03 and 2017ZZ10).
Conflict of interest
The authors declare that they had no conflict of interest.
The animal experiment was approved by the First Affiliated Hospital, School of Medicine, Zhejiang University (No. 2015-15).
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