Isolation and molecular characterization of an H5N1 swine influenza virus in China in 2015

  • Haibo Wu
  • Fan Yang
  • Rufeng Lu
  • Lihua Xu
  • Fumin Liu
  • Xiuming Peng
  • Nanping Wu
Brief Report

Abstract

In 2015, an H5N1 influenza virus was isolated from a pig in Zhejiang Province, Eastern China. This strain was characterized by whole-genome sequencing with subsequent phylogenetic analysis. Phylogenetic analysis showed that all segments from this strain belonged to clade 2.3.2 and that it had received its genes from poultry influenza viruses in China. A Glu627Lys mutation associated with pathogenicity was observed in the PB2 protein. This strain was moderately pathogenic in mice and was able to replicate without prior adaptation. These results suggest that active surveillance of swine influenza should be used as an early warning system for influenza outbreaks in mammals.

Keywords

Swine influenza virus H5N1 Reassortment Poultry EASTERN China 

Notes

Compliance with ethical standards

Funding

This study was funded by Grants from the National Science Foundation of the People’s Republic of China (81502852), Zhejiang Provincial Natural Science Foundation of China (LY15H190003), and the Independent Task of State Key Laboratory for Diagnosis and Treatment of Infectious Diseases (Nos. 2016ZZ03 and 2017ZZ10).

Conflict of interest

The authors declare that they had no conflict of interest.

Ethical approval

The animal experiment was approved by the First Affiliated Hospital, School of Medicine, Zhejiang University (No. 2015-15).

Supplementary material

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2017

Authors and Affiliations

  • Haibo Wu
    • 1
  • Fan Yang
    • 1
  • Rufeng Lu
    • 2
  • Lihua Xu
    • 3
  • Fumin Liu
    • 1
  • Xiuming Peng
    • 1
  • Nanping Wu
    • 1
  1. 1.State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of MedicineZhejiang UniversityHangzhouChina
  2. 2.Department of Emergencythe First Affiliated Hospital of Zhejiang Chinese Medical UniversityHangzhouChina
  3. 3.Animal Husbandry and Veterinary InstituteZhejiang Academy of Agricultural ScienceHangzhouChina

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