Archives of Virology

, Volume 163, Issue 3, pp 761–765 | Cite as

Molecular characterization of a novel luteovirus infecting apple by next-generation sequencing

  • Pan Shen
  • Xin Tian
  • Song Zhang
  • Fang Ren
  • Ping Li
  • Yun-qi Yu
  • Ruhui Li
  • Changyong Zhou
  • Mengji Cao
Annotated Sequence Record


A new single-stranded positive-sense RNA virus, which shares the highest nucleotide (nt) sequence identity of 53.4% with the genome sequence of cherry-associated luteovirus South Korean isolate (ChALV-SK, genus Luteovirus), was discovered in this work. It is provisionally named apple-associated luteovirus (AaLV). The complete genome sequence of AaLV comprises 5,890 nt and contains eight open reading frames (ORFs), in a very similar arrangement that is typical of members of the genus Luteovirus. When compared with other members of the family Luteoviridae, ORF1 of AaLV was found to encompass another ORF, ORF1a, which encodes a putative 32.9-kDa protein. The ORF1-ORF2 region (RNA-dependent RNA polymerase, RdRP) showed the greatest amino acid (aa) sequence identity (59.7%) to that of cherry-associated luteovirus Czech Republic isolate (ChALV-CZ, genus Luteovirus). The results of genome sequence comparisons and phylogenetic analysis, suggest that AaLV should be a member of a novel species in the genus Luteovirus. To our knowledge, it is the sixth member of the genus Luteovirus reported to naturally infect rosaceous plants.



This research was supported by the National Natural Science Foundation of China (31501611), Chongqing Research Program of Basic Research and Frontier Technology (cstc2017jcyjBX0016), Fundamental Research Funds for the Central Universities (XDJK2016B021, SWU116012), Special Fund for Agro-Scientific Research in the Public Interest (201203076-01), and the Innovation Foundation of Chongqing City for Postgraduate (CYS17071).

Compliance with ethical standards

Ethical standards

I have read and have abided by the statement of ethical standards for manuscripts submitted to Archives of Virology.

Conflict of interest

All authors declare they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animal performed by any of the authors.

Supplementary material

705_2017_3633_MOESM1_ESM.txt (6 kb)
Supplementary material 1 (TXT 5 kb)
705_2017_3633_MOESM2_ESM.doc (410 kb)
Supplementary material 2 (DOC 410 kb)


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Copyright information

© Springer-Verlag GmbH Austria 2017

Authors and Affiliations

  1. 1.National Citrus Engineering Research Center, Citrus Research InstituteSouthwest UniversityChongqingChina
  2. 2.Academy of Agricultural Sciences, Southwest UniversityChongqingChina
  3. 3.Research Institute of PomologyChinese Academy of Agricultural SciencesXingchengChina
  4. 4.USDA-ARS, National Germplasm Resources LaboratoryBeltsvilleUSA

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