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Quantitative analysis of senecavirus A in tissue samples from naturally infected newborn piglets

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Abstract

In this study, we determined the distribution of senecavirus A (SVA) and viral RNA load in different organs and tissues of naturally infected piglets. A TaqMan-based qRT-PCR assay was performed using RNA extracted from brainstem, cerebellum, cerebrum, heart, kidney, liver, lungs, small intestine, spleen, urinary bladder, and tonsils of seven newborn piglets. SVA was detected in 57 out of 70 tissue samples (81.4%). Viral loads ranged from 4.07 to 10.38 log10 genomic copies per g of tissue. The results show that SVA has tropism for various organs in naturally infected newborn piglets, especially for tonsils, spleen, lungs, and liver. Lymphoid organs had the highest viral loads and may be important sites for SVA replication.

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Acknowledgements

The authors thank the following Brazilian Institutes for financial support: CNPq, CAPES, FINEP, and FAP/PR. Alfieri, AF, Alfieri, AA, Headley, S.A., Oliveira TES, Leme, RA, and Dall Agnol, AM are recipients of CNPq fellowships.

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Authors and Affiliations

  1. Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Campus Universitário, PO Box 10011, Paraná, CEP, 86057-970, Brazil

    Alais Maria Dall Agnol, Flavia Megumi Miyabe, Raquel Arruda Leme, Amauri Alcindo Alfieri & Alice Fernandes Alfieri

  2. Laboratory of Animal Pathology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Campus Universitário, PO Box 10011, Paraná, CEP, 86057-970, Brazil

    Thalita Evani Silva Oliveira & Selwyn Arlington Headley

  3. Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Campus Universitário, PO Box 10011, Paraná, CEP, 86057-970, Brazil

    Alais Maria Dall Agnol, Flavia Megumi Miyabe, Raquel Arruda Leme, Amauri Alcindo Alfieri & Alice Fernandes Alfieri

Authors
  1. Alais Maria Dall Agnol
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  2. Flavia Megumi Miyabe
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  3. Raquel Arruda Leme
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  4. Thalita Evani Silva Oliveira
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  5. Selwyn Arlington Headley
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  6. Amauri Alcindo Alfieri
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  7. Alice Fernandes Alfieri
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Corresponding author

Correspondence to Amauri Alcindo Alfieri.

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Conflict of interest

The authors declared no potential conflicts of interest relative to the research, authorship, and/or publication of this article.

Funding

This study was funded by CNPq (Grant number 150346/2017-4).

Ethical approval

The study was submitted to the Ethics Committee on Animal Experiments of the Universidade Estadual de Londrina and approved under the identification number 11363.2015.16. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Handling Editor: Zhenhai Chen.

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Dall Agnol, A.M., Miyabe, F.M., Leme, R.A. et al. Quantitative analysis of senecavirus A in tissue samples from naturally infected newborn piglets. Arch Virol 163, 527–531 (2018). https://doi.org/10.1007/s00705-017-3630-8

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  • DOI: https://doi.org/10.1007/s00705-017-3630-8

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