Significant differences in the intra-host genetic diversity of Helicoverpa armigera nucleopolyhedrovirus dnapol after serial in vivo passages in the same insect population
A denaturing gradient gel electrophoresis assay was used to assess the genetic diversity within a region of the DNA polymerase gene (dnapol) in Helicoverpa armigera nucleopolyhedrovirus (HearNPV) populations over serial in vivo passages. There was no evidence of movement towards a consensus dnapol variant composition in the different host larvae after multiple per os passages. The study showed that the HearNPV variant structure after in vivo passages in the same host population is not necessarily convergent, and that it may be reasonable to expect significant differences in intra-host HearNPV genetic diversity after inoculation of larvae with a genotypically-diverse HearNPV inoculum.
We appreciate the support received from the University of the Witwatersrand, Johannesburg. We thank anonymous reviewers for comments that improved this manuscript.
Compliance with ethical standards
This study was partially funded by the Research Incentive Programme of the University of the Witwatersrand, Johannesburg.
Conflict of interest
Gustav Bouwer declares that he has no conflict interest. Dale Kitchin declares that he has no conflict interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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