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Molecular characterization of a novel fusarivirus infecting the plant-pathogenic fungus Neofusicoccum luteum

Archives of Virology volume 163, pages 559–562 (2018)Cite this article

Abstract

Double-stranded RNAs from an isolate of Neofusicoccum luteum collected from grapevines were analyzed by high-throughput sequencing. Contig annotations revealed the presence of a potential novel virus belonging to the newly proposed family Fusariviridae. Completion of the viral genome sequence was performed. The genome is 6,244 nucleotide long, excluding the poly(A) tail and contains two putative open reading frames (ORFs). The first one encodes a large polypeptide of 1,552 amino acids (aa) with conserved RNA-dependent RNA polymerase and helicase domains typical of viral replicases. The second ORF encodes a putative 475-aa-long polypeptide showing weak homology to the corresponding ORF of Macrophomina phaseolina single-stranded RNA virus 1, for which no function is known so far. Phylogenetic analyses indicated that this virus should be considered a novel mycovirus belonging to the proposed family Fusariviridae, for which the name “Neofusicoccum luteum fusarivirus 1” (NlFV1) is proposed.

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References

  1. Wunderlich N, Ash G, Steel C, Rahman H, Savocchia S (2010) Botryosphaeriaceae associated with bunch rot of grapes in South Eastern Australia. Phytopathol Mediterr 49:106

    Google Scholar 

  2. Yacoub A, Gerbore J, Magnin N, Chambon P, Dufour MC, Corio-Costet MF, Guyoneaud P, Rey P (2016) Ability of Pythium oligandrum strains to protect Vitis vinifera against Phaeomoniella chlamydospora, a pathogen involved in Esca, by inducing plant resistance. Biol Control 92:7–16

    Article  Google Scholar 

  3. Ghabrial SA, Castón JR, Jiang D, Nibert ML, Suzuki N (2015) 50-plus years of fungal viruses. Virology 479–480:356–368

    Article  PubMed  Google Scholar 

  4. Wang LP, Jiang JJ, Wang YF, Hong N, Zhang F, Xu WX, Wang GP (2014) Hypovirulence of the phytopathogenic fungus Botryosphaeria dothidea: association with a coinfecting Chrysovirus and a Partitivirus. J Virol 88:7517–7527

    Article  PubMed  PubMed Central  Google Scholar 

  5. Zhai L, Xiang J, Zhang M, Fu M, Yang Z, Hong N, Wang G (2016) Characterization of a novel double-stranded RNA mycovirus conferring hypovirulence from the phytopathogenic fungus Botryosphaeria dothidea. Virology 493:75–85

    CAS  Article  PubMed  Google Scholar 

  6. Phillips AJL (2002) Botryosphaeria species associated with diseases of grapevines in Portugal. Phytopathol Mediterr 41:3–18

    Google Scholar 

  7. Marais A, Nivault A, Faure C, Theil S, Comont G, Candresse T, Corio-Costet MF (2017) Determination of the complete genomic sequence of Neofusicoccum luteum mitovirus 1 (NLMV1), a novel mitovirus associated with a phytopathogenic Botryosphaeriaceae. Arch Virol 162:2477–2480

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  8. Zhang R, Liu S, Chiba S, Kondo H, Kanematsu S, Suzuki N (2014) A novel single-stranded RNA virus isolated from a phytopathogenic filamentous fungus, Rosellinia necatrix, with similarity to hypo-like viruses. Front Microbiol 5:e360

    Google Scholar 

  9. Zhong J, Zhao SQ, Li GF, Pang XD, Deng XJ, Zhu HJ, Gao BD, Zhang Q (2016) A novel fusarivirus isolated from the phytopathogenic fungus Nigrospora oryzae. Virus Genes 52:891–895

    CAS  Article  PubMed  Google Scholar 

  10. Bellée A, Comont G, Nivault A, Abou-Mansour E, Coppin C, Dufour MC, Corio-Costet MF (2017) Life traits of four Botryosphaeriacea species and molecular responses of different genotypes and cultivar of Vitis vinifera. Plant Pathol 66:763–776

    Article  Google Scholar 

  11. Marzano SL, Nelson BD, Ajayi-Oyetunde O, Bradley CA, Hughes TJ, Hartman GL, Eastburn DM, Domier LL (2016) Identification of diverse mycoviruses through metatranscriptomics: characterization of the viromes of five major fungal plant pathogens. J Virol 90:6846–6863

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  12. Liu R, Cheng J, Fu Y, Jiang D, Xie J (2015) Molecular characterization of a novel positive-sense, single-stranded RNA mycovirus infecting the plant pathogenic fungus Sclerotinia sclerotiorum. Viruses 7:2470–2484

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  13. Zhong J, Shang HH, Zhu CX, Zhu JZ, Zhu HJ, Hua Y, Gao BD (2016) Characterization of a novel single-stranded RNA virus, closely related to fusariviruses, infecting the plant pathogenic fungus Alternaria brassicicola. Virus Res 217:1–7

    CAS  Article  PubMed  Google Scholar 

  14. Chu YM, Jeon JJ, Yea SJ, Kim YH, Yun SH, Lee YW, Kim KH (2002) Double-stranded RNA mycovirus from Fusarium graminearum. Appl Environ Microbiol 68:2529–2534

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  15. Nerva L, Ciuffo M, Vallino M, Margaria P, Varese GC, Gnavi G, Turina M (2016) Multiple approaches for the detection and characterization of viral and plasmid symbionts from a collection of marine fungi. Virus Res 219:22–38

    CAS  Article  PubMed  Google Scholar 

  16. Osaki H, Sasaki A, Nomiyama K, Tomioka K (2016) Multiple virus infection in a single strain of Fusarium poae shown by deep sequencing. Virus Genes 52:835–847

    CAS  Article  PubMed  Google Scholar 

Download references

Acknowledgements

The authors thank the Platform Genotoul (INRA, Toulouse, France) for the Illumina sequencing.

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Affiliations

  1. INRA, UMR 1332 Biologie du Fruit et Pathologie, University of Bordeaux, 71 Avenue Edouard Bourlaux, CS 20032, 33882, Villenave d’Ornon Cedex, France

    Armelle Marais, Chantal Faure, Sébastien Theil & Thierry Candresse

  2. INRA, UMR 1065 Santé et Agroécologie du Vignoble, Bordeaux Sciences Agro, 71 Avenue Edouard Bourlaux, CS 20032, 33882, Villenave d’Ornon Cedex, France

    Aurélia Nivault, Gwenaelle Comont & Marie-France Corio-Costet

Authors
  1. Armelle Marais
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  2. Aurélia Nivault
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  3. Chantal Faure
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  4. Gwenaelle Comont
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  5. Sébastien Theil
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  6. Thierry Candresse
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  7. Marie-France Corio-Costet
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Corresponding author

Correspondence to Armelle Marais.

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All authors declare no conflict of interest.

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This article does not contain any studies with animals or human participants performed by any of the authors.

Additional information

The nucleotide sequence reported in this study has been deposited in GenBank under the accession number KY906213.

Handling Editor: Robert H.A. Coutts.

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Marais, A., Nivault, A., Faure, C. et al. Molecular characterization of a novel fusarivirus infecting the plant-pathogenic fungus Neofusicoccum luteum . Arch Virol 163, 559–562 (2018). https://doi.org/10.1007/s00705-017-3620-x

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  • DOI: https://doi.org/10.1007/s00705-017-3620-x