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Rapid visual detection of lily mottle virus using a loop-mediated isothermal amplification method

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Abstract

Lily mottle virus (LMoV; genus Potyvirus, family Potyviridae) infects plants of the genus Lilium, causing a reduction in flower and bulb quality. A rapid and sensitive loop-mediated isothermal amplification (LAMP) assay was developed to detect the coat protein gene of LMoV. This LAMP method was highly specific for LMoV, with no cross-reaction with other lily viruses. The sensitivity of LMoV using the LAMP assay was 100 times more sensitive than that using conventional polymerase chain reaction. A reverse transcription LAMP (RT-LAMP) was then successfully applied to detect LMoV RNA. The newly established LAMP and one-step RT-LAMP provide an alternative method for detecting LMoV in lily plants.

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Author information

Author notes

  1. Binan Zhao and Dan Yang equal contributors.

Authors and Affiliations

  1. Shanghai Academy of Agricultural Sciences, Shanghai, People’s Republic of China

    Yongchun Zhang, Xiao Zhao, Bin Shi, Youming Cai & Kai Zhao

  2. Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences, Shanghai, People’s Republic of China

    Xiao Zhao & Kai Zhao

  3. College of Life and Environment Sciences, Shanghai Normal University, Shanghai, People’s Republic of China

    Yan Xu, Xiaorui Fan, Yanan Du, Qi Zhang & Xiaoxia Zhang

  4. Department of Clinical Medicine, XuZhou Medical University, Xuzhou, People’s Republic of China

    Binan Zhao

  5. School of Life science, Taizhou University, Taizhou, People’s Republic of China

    Dan Yang, Jieling Liang & Ziping Zhu

Authors
  1. Binan Zhao
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  2. Dan Yang
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  3. Yongchun Zhang
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  4. Yan Xu
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  5. Xiao Zhao
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  6. Jieling Liang
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  7. Xiaorui Fan
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  8. Yanan Du
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  9. Ziping Zhu
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  10. Bin Shi
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  11. Qi Zhang
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  12. Xiaoxia Zhang
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  13. Youming Cai
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  14. Kai Zhao
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Contributions

KZ, BZ and DY participated in the design and carried out the majority of the experiments and drafted, reviewed and edited the manuscript. YZ, YX, XZ, JL, XF, QZ and XZ helped to perform the experiments. BS contributed reagents/materials/analysis tools, YC, YZ and ZZ participated in its design and coordination. YD edited the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Youming Cai or Kai Zhao.

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Conflict of interest

The authors declare that they have no conflict of interest.

Funding

This work was financially supported by the Shanghai Agriculture Applied Technology Development Program, China (Grant No. G2014070203) and the Agriculture Research System of Shanghai, China (Grant No. 201708). These funding bodies had no role in design, collection, or analysis of the study or preparation of the manuscript.

Additional information

Handling Editor: Ralf Georg Dietzgen.

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Zhao, B., Yang, D., Zhang, Y. et al. Rapid visual detection of lily mottle virus using a loop-mediated isothermal amplification method. Arch Virol 163, 545–548 (2018). https://doi.org/10.1007/s00705-017-3618-4

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  • DOI: https://doi.org/10.1007/s00705-017-3618-4

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