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Regulation of the chikungunya-virus-induced innate inflammatory response by protein tyrosine phosphatase non-receptor 6 in muscle cells

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Abstract

Chikungunya virus (CHIKV)-induced myositis is an emerging affliction with high incidence globally. Given the essential regulatory role of protein tyrosine phosphatase non-receptor 6 (PTPN6) in virus-induced myositis, the expression of the PTPN6 and TNF-α genes in a CHIKV-infected muscle cell line was examined by quantitative PCR, and the expression of PTPN6 and STAT 3 was examined by immunoblotting. In addition, the effect of PTPN6 siRNA treatment on TNF-α gene expression was assessed. Increased higher expression of PTPN6 and TNF-α, and significant upregulation of TNF-α upon PTPN6 siRNA treatment were observed, suggesting that CHIKV has the ability to induce host PTPN6 gene expression, which may lead to a decreased pro-inflammatory immune response in the host.

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Acknowledgements

We thank the Director, National Institute of Virology, for all the encouragements. Mr. Subrat Thanapati would like to thank the University Grant Commission, New Delhi, India, for providing the Senior Research Fellowship.

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Authors and Affiliations

  1. Hepatitis Group, National Institute of Virology, 130/1, Sus Road, Pashan, Pune, Maharashtra, 411021, India

    Subrat Thanapati, Shruti P. Kulkarni & Anuradha S. Tripathy

  2. Entomology group, National Institute of Virology, 130/1, Sus Road, Pashan, Pune, Maharashtra, 411021, India

    A. B. Sudeep

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  1. Subrat Thanapati
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  3. Shruti P. Kulkarni
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Correspondence to Anuradha S. Tripathy.

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This research did not receive any specific Grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Handling Editor: Patricia Aguilar.

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Thanapati, S., Sudeep, A.B., Kulkarni, S.P. et al. Regulation of the chikungunya-virus-induced innate inflammatory response by protein tyrosine phosphatase non-receptor 6 in muscle cells. Arch Virol 163, 243–248 (2018). https://doi.org/10.1007/s00705-017-3615-7

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  • DOI: https://doi.org/10.1007/s00705-017-3615-7

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