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Sequence analysis of six full-length bean yellow mosaic virus genomes reveals phylogenetic diversity in India strains, suggesting subdivision of phylogenetic group-IV

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Abstract

We report the complete genome sequence of five bean yellow mosaic virus (BYMV) isolates (CK-GL1, CK-GL3, CK-GL4, CK-GL5 and Vfaba2) that share 74.6-98.9% (nucleotide) and 81.5-99.1% (amino acid) identity with globally available BYMV sequences. Phylogenetic analysis clustered them specifically in BYMV phylogenetic group-IV within the existing nine groups. The CK-GL1, CK-GL2, CK-GL4 and CK-GL5 isolates formed a discrete cluster within group-IV. The present study suggests subdivision of group-IV into subgroup-IVa and IVb. Moreover, infectivity assays using in vitro RNA transcripts from subgroup-IVa (CK-GL3 isolate) and IVb (CK-GL1 isolate) showed distinct biological differences between the isolates supporting subdivision.

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Acknowledgements

Thanks are due to the Director, CSIR-NBRI, Lucknow for access to facilities. Author C. Kaur is thankful to the University Grants Commission, India for her fellowship.

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Authors and Affiliations

  1. Plant Molecular Virology Laboratory, CSIR-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, Uttar Pradesh, 226 001, India

    Charanjeet Kaur, Rashmi Raj, Susheel Kumar & Shri Krishna Raj

  2. Department of Plant Microbe Interactions, CSIR-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, Uttar Pradesh, 226 001, India

    Rashmi Raj & Susheel Kumar

  3. Amity Institute of Virology and Immunology, Amity University, Sector 125, Noida, Uttar Pradesh, 201 313, India

    Ashish Srivastava

Authors
  1. Charanjeet Kaur
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  2. Rashmi Raj
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  4. Susheel Kumar
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  5. Shri Krishna Raj
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Correspondence to Susheel Kumar or Shri Krishna Raj.

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The present study is partially funded by CSIR funded PMSI project “BSC0117”.

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The authors declare no conflict of interest.

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This article does not contain any studies with human participants performed by any of the authors.

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Not applicable as this article does not contain any study with human participants performed by any of the authors.

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Handling Editor: Ralf Georg Dietzgen.

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Kaur, C., Raj, R., Srivastava, A. et al. Sequence analysis of six full-length bean yellow mosaic virus genomes reveals phylogenetic diversity in India strains, suggesting subdivision of phylogenetic group-IV. Arch Virol 163, 235–242 (2018). https://doi.org/10.1007/s00705-017-3609-5

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  • DOI: https://doi.org/10.1007/s00705-017-3609-5

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