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Archives of Virology

, Volume 163, Issue 3, pp 751–754 | Cite as

Complete genome sequence of a lettuce chlorosis virus isolate from China and genome recombination/rearrangement analysis

Annotated Sequence Record

Abstract

We determined the complete genome sequence of a lettuce chlorosis crinivirus (LCV) from China (LCV-NJ). The bipartite genome of LCV-NJ consists of RNA1 and RNA2 which are 8165 and 8454 nucleotides (nt) in length, respectively. The genomic structure of LCV-NJ RNA1 resembles that of LCV-California, an isolate with four open reading frames (ORFs) in RNA1. Although the amino acid sequences of ORF 1a and 1b have 92 and 99% identity between LCV-NJ and LCV-California, ORF 2 and ORF3 of LCV-NJ share only 63 and 71% identity with those of LCV-California, respectively. In addition LCV-NJ RNA2 contains 9 ORFs, compared to 10 ORFs in LCV-California. ORF10 was missing due to the deletion of a 173-nt sequence within the 3’-terminal region of LCV-NJ RNA2. Insertion or deletion of sequences of varying lengths was also observed in RNA1 and other regions of RNA2. Based on these findings, we propose that LCV-NJ/LCV-California may have undergone genome recombination and/or rearrangement in RNA1 and RNA2.

Notes

Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (31501607 and 31471746), the Special Fund for Agro-Scientific Research in the Public Interest (201303028), the Youth Talent Support Program of China and Distinguished Professor of Jiangsu Province to X. T., the Fundamental Research Funds for the Central Universities (KYTZ201403, KJQN201661).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

This article does not contain any studies with human participants.

Supplementary material

705_2017_3604_MOESM1_ESM.pdf (306 kb)
Supplementary material 1 (PDF 306 kb)
705_2017_3604_MOESM2_ESM.pdf (170 kb)
Supplementary material 2 (PDF 169 kb)

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Copyright information

© Springer-Verlag GmbH Austria 2017

Authors and Affiliations

  1. 1.Department of Plant PathologyNanjing Agricultural UniversityNanjingChina
  2. 2.Department of Plant Pathology and Plant-Microbe BiologyCornell UniversityIthacaUSA

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