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Diversity and evolution analysis of glycoprotein GP85 from avian leukosis virus subgroup J isolates from chickens of different genetic backgrounds during 1989-2016: Coexistence of five extremely different clusters

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Abstract:

ALV-J has caused the most serious losses to the poultry industry in China. The gp85-coding sequence of ALV-J is known to be prone to mutation, but any association between the gp85 gene and breed of chicken remains unclear. A comprehensive and systematic study of the evolutionary process of ALV-J in China is needed. In this study, we compared and analyzed gp85 gene sequences from 198 ALV-J isolates, originating from China, USA, UK and France during 1989-2016. These were sorted into five clusters. Cluster 1, 2, 3, 4 and 5 included isolates from chicken types of different genetic backgrounds, e.g. white-feather broiler, Guangxi indigenous chicken breeds, Yellow chickens and layer chickens respectively. A correlation comparison of amino acid sequence similarities in the gp85 protein among the five clusters showed significant differences (P < 0.01) with the exception being when the third and fifth cluster were compared (P > 0.05). Results of entropy analysis of the gp85 sequences revealed that cluster 3 had the largest variation and cluster 1 had the least variation. The N-glycosylation sites in the majority of isolates numbered 14, 16, 17, 16 and 16, respectively, with regards to clusters 1-5. In addition, 5 isolates from cluster 3 had one more glycosylation site than the other isolates from cluster 3. Our study provides evidence that there were five extremely different ALV-J clusters during 1989-2016 and that the gp85 genes isolated from indigenous chicken breed isolates had the largest variation.

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Acknowledgements

This work was supported by the National Eradication Program for AL [201203055], the Guangxi Program for Modern Agricultural Industry Technical System Construction-Chicken Industry [nycytxgxcxtd-04-20-2], and the Innovation Project of Guangxi Graduate Education [T3340098003]. The manuscript was kindly reviewed by Dr. Richard Roberts, Aurora, CO 80014, USA.

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Authors and Affiliations

  1. Institute for Poultry Science and Health, Guangxi University, Nanning, 530004, China

    Peikun Wang, Lulu Lin, Haijuan Li, Yongli Yang, Teng Huang & Ping Wei

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  1. Peikun Wang
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  2. Lulu Lin
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  3. Haijuan Li
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  4. Yongli Yang
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Correspondence to Ping Wei.

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Author Peikun Wang declares that he has no conflict of interest; Author Lulu Lin declares that she has no conflict of interest; Author Haijuan Li declares that she has no conflict of interest; Author Yongli Yang declares that she has no conflict of interest; Author Teng Huang declares that he has no conflict of interest; Author Ping Wei declares that he has no conflict of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Handling editor: Zhenhai Chen.

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Wang, P., Lin, L., Li, H. et al. Diversity and evolution analysis of glycoprotein GP85 from avian leukosis virus subgroup J isolates from chickens of different genetic backgrounds during 1989-2016: Coexistence of five extremely different clusters. Arch Virol 163, 377–389 (2018). https://doi.org/10.1007/s00705-017-3601-0

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  • DOI: https://doi.org/10.1007/s00705-017-3601-0

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