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Complete genome sequence of datura leaf curl virus, a novel begomovirus infecting Datura innoxia in Sudan, related to begomoviruses causing tomato yellow leaf curl disease

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Abstract

Several species in the genus Datura (family Solanaceae) are hosts for begomoviruses (family Geminiviridae), both in the New World and the Old World. Here, we report the genome characterization of two isolates of a novel Old World monopartite begomovirus infecting Datura innoxia in Sudan, which we propose to be named “datura leaf curl virus” (DaLCV). Pairwise sequence comparisons and phylogenetic analysis showed that DaLCV isolates are related to begomoviruses causing tomato yellow leaf curl disease, a global menace for tomato crops. Thus, the novel begomovirus could pose an additional threat to tomato cultivation in Africa.

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References

  1. Brown JK, Zerbini FM, Navas-Castillo J, Moriones E, Ramos-Sobrinho R, Silva JCF, Fiallo-Olivé E, Briddon RW, Hernández-Zepeda C, Idris A, Malathi VG, Martin DP, Rivera-Bustamante R, Ueda S, Varsani A (2015) Revision of Begomovirus taxonomy based on pairwise sequence comparisons. Arch Virol 160:1593–1619

    Article  CAS  PubMed  Google Scholar 

  2. Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. El-Massad HA, Satti AA, Alabjar ZA (2012) Insecticidal potentiality of Datura innoxia leaf extracts against the cluster bug (Agonoscelis pubescens (Thunberg). Glo Adv Res J Environ Sci Toxicol 1:172–177

    Google Scholar 

  4. Fourment M, Gibbs AJ, Gibbs MJ (2008) SWeBLAST: a sliding window web-based BLAST tool for recombinant analysis. J Virol Methods 152:98–101

    Article  CAS  PubMed  Google Scholar 

  5. Haible D, Kober S, Jeske H (2006) Rolling circle amplification revolutionizes diagnosis and genomics of geminiviruses. J Virol Methods 135:9–16

    Article  CAS  PubMed  Google Scholar 

  6. Hollingsworth PM, Forrest LL, Spouge JL et al (2009) A DNA barcode for land plants. Proc Natl Acad Sci USA 106:12794–12797

    Article  CAS  PubMed Central  Google Scholar 

  7. Inoue-Nagata AK, Albuquerque LC, Rocha WB, Nagata T (2004) A simple method for cloning the complete begomovirus genome using the bacteriophage φ29 DNA polymerase. J Virol Methods 116:209–211

    Article  CAS  PubMed  Google Scholar 

  8. Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    Article  CAS  PubMed  Google Scholar 

  9. Martin DP, Murrell B, Golden M, Khoosal A, Muhire B (2015) RDP4: Detection and analysis of recombination patterns in virus genomes. Virus Evol 1:vev003

    Article  PubMed  PubMed Central  Google Scholar 

  10. Mohammed HS, El Siddig MA, El Hussein AA, Navas-Castillo J, Fiallo-Olivé E (2017) First report of Datura innoxia as a natural host of Watermelon chlorotic stunt virus in Sudan. Plant Dis 101:1334

    Article  Google Scholar 

  11. Muhire B, Varsani A, Martin DP (2014) SDT: a virus classification tool based on pairwise sequence alignment and identity calculation. PLoS One 9:e108277

    Article  PubMed  PubMed Central  Google Scholar 

  12. Navas-Castillo J, Fiallo-Olivé E, Sánchez-Campos S (2011) Emerging virus diseases transmitted by whiteflies. Annu Rev Phytopatol 49:219–248

    Article  CAS  Google Scholar 

  13. Schmelzer GH, Gurib-Fakim A (2008) Plant resources of tropical Africa 11 (1). Medicinal plants 1. PROTA Foundation, Wageningen, Netherlands

  14. Wyatt SD, Brown JK (1996) Detection of subgroup III geminivirus isolates in leaf extracts by degenerate primers and polymerase chain reaction. Phytopathology 86:1288–1293

    Article  Google Scholar 

  15. Zerbini FM, Briddon RW, Idris A, Martin DP, Moriones E, Navas-Castillo J, Rivera-Bustamante R, Roumagnac P, Varsani A, ICTV Report Consortium (2017) ICTV virus taxonomy profile: Geminiviridae. J Gen Virol 98:131–133

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Botany Department, Faculty of Science, University of Khartoum, P.O. Box 321, 11115, Khartoum, Sudan

    Heyam S. Mohammed, Marmar A. El Siddig & Adil A. El Hussein

  2. Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, Universidad de Málaga - Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Avenida Dr. Wienberg s/n, 29750, Algarrobo-Costa, Málaga, Spain

    Heyam S. Mohammed, Jesús Navas-Castillo & Elvira Fiallo-Olivé

Authors
  1. Heyam S. Mohammed
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  2. Marmar A. El Siddig
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  3. Adil A. El Hussein
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  4. Jesús Navas-Castillo
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  5. Elvira Fiallo-Olivé
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Corresponding author

Correspondence to Elvira Fiallo-Olivé.

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Funding

This work was supported by grants AGL2013-48913-C2-1-R and AGL2016-75819-C2-2-R from Ministerio de Economía y Competitividad (MINECO, Spain) co-financed by the European Regional Development Fund. E.F.O. is recipient of a “Juan de la Cierva-Incorporación” contract from MINECO, Spain. The financial support of the Ministry of Higher Education and Scientific Research of Sudan is also acknowledged.

Conflict of interest

The authors declare that they have no conflict of interest.

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No studies involving human participants or animals performed by any of the authors are described in this article.

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Handling Editor: F. Murilo Zerbini.

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Mohammed, H.S., El Siddig, M.A., El Hussein, A.A. et al. Complete genome sequence of datura leaf curl virus, a novel begomovirus infecting Datura innoxia in Sudan, related to begomoviruses causing tomato yellow leaf curl disease. Arch Virol 163, 273–275 (2018). https://doi.org/10.1007/s00705-017-3574-z

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  • DOI: https://doi.org/10.1007/s00705-017-3574-z

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