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Dicipivirus (family Picornaviridae) in wild Northern white-breasted hedgehog (Erinaceus roumanicus)

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Abstract

Using random amplification and high-throughput sequencing technology a novel picornavirus with dicistronic genome organization and genetically related to canine picodicistrovirus (genus Dicipivirus, family Picornaviridae) was identified and characterized in Northern white-breasted hedgehogs. Hedgehog dicipivirus (hedgehog/H14/2015/HUN, MF188967) was detected in 15 (75%) of 20 faecal specimens by RT-PCR with high viral loads (up to 8.2x108 genomic copies/ml faeces). Hedgehog dicipivirus RNA was also identified in blood, ear skin, abdominal muscle and liver tissues. While the general dicistronic genome organization of hedgehog/H14/2015/HUN is similar to canine picodicistrovirus (5’UTR-P1-IGR-P2/P3-3UTR) there are some unique genome characteristics within the untranslated regions, especially in the functional IRES elements. This study reports the putative second member of the genus Dicipivirus, in a novel host species.

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Acknowledgements

This work was supported by a Grant from the Hungarian Scientific Research Fund (OTKA/NKFIH K111615), by the Hungarian Nature Research Society and by NHLBI R01-HL105770. P.P., Á.B. and G.F. were supported by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences. The GenBank [/EMBL/DDBJ] accession number for the study sequence: MF188967.

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Authors and Affiliations

  1. Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric Viruses, ÁNTSZ Regional Institute of State Public Health Service, Pécs, Hungary

    Gábor Reuter, Ákos Boros & Péter Pankovics

  2. Department of Medical Microbiology and Immunology, Medical Center, University of Pécs, Szigeti út 12, Pécs, 7624, Hungary

    Gábor Reuter, Ákos Boros & Péter Pankovics

  3. Department of Parasitology and Zoology, University of Veterinary Medicine, Budapest, Hungary

    Gábor Földvári & Sándor Szekeres

  4. Hungarian Nature Research Society (HuNaReS), Ajka, Hungary

    Róbert Mátics

  5. Department of Nature Conservation, Kaposvár University, Kaposvár, Hungary

    Róbert Mátics

  6. Blood Systems Research Institute, San Francisco, CA, USA

    Beatrix Kapusinszky & Eric Delwart

  7. University of California, San Francisco, CA, USA

    Eric Delwart

Authors
  1. Gábor Reuter
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  2. Ákos Boros
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  3. Gábor Földvári
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  4. Sándor Szekeres
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  5. Róbert Mátics
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  6. Beatrix Kapusinszky
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  7. Eric Delwart
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  8. Péter Pankovics
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Corresponding author

Correspondence to Gábor Reuter.

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Funding

This study was funded by the Hungarian Scientific Research Fund (OTKA/NKFIH K111615) and by the Hungarian Nature Research Society and NHLBI R01-HL105770.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Handling Editor: Diego G. Diel.

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Reuter, G., Boros, Á., Földvári, G. et al. Dicipivirus (family Picornaviridae) in wild Northern white-breasted hedgehog (Erinaceus roumanicus). Arch Virol 163, 175–181 (2018). https://doi.org/10.1007/s00705-017-3565-0

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  • DOI: https://doi.org/10.1007/s00705-017-3565-0

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