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Binding of rabbit hemorrhagic disease virus-like particles to host histo-blood group antigens is blocked by antisera from experimentally vaccinated rabbits

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Abstract

During infection host histo-blood group antigens (HBGAs) act as attachment factors that interact with rabbit hemorrhagic disease virus (RHDV) and participate in the infectious process. In the present study, baculovirus expressing recombinant RHDV capsid protein (VP60r) as a vaccine immunogen was used to test its antigenicity and immunogenicity via immunization experiments. Each group of rabbits immunized with VP60r was found to be fully protected against RHDV challenge. The duration of immunity of the vaccine following the inoculation of a single dose was determined to be at least 240 days. RHDV-specific humoral responses in antisera from inoculated rabbits were analyzed using VP60r virus-like particle (VLP)-based ELISA. Anti-VP60-specific antibody was produced by 7 days post-primary immunization. Following this stage, the levels of this antibody increased steadily, peaking at 90 days and maintaining a high level until 240 days. We developed a synthetic carbohydrate assay to detect blockage in attachment of RHDV VLPs to HBGAs by the rabbit antisera. On day 7 post-immunization, serum samples were demonstrated to block the binding of H type 2 to RHDV VLPs, with a blocking rate of almost 60%, a value that then increased steadily over time. From day 60 to day 240 post-immunization, serum samples completely blocked the binding of H type 2 to RHDV VLPs, with a blocking rate of almost 100%. This indicated that VP60-induced antibodies neutralize the interaction of RHDV with HBGAs.

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Acknowledgements

The project was supported by the National Natural Sciences Foundation of China (31502073) and the Jiangsu Province Natural Sciences Foundation (BK20140740), as well as by the earmarked fund for China Agriculture Research System (No. [CARS-44-C-1]) and Agroscientific Research in the Public Interest (201303046). The authors are grateful to the staff of the Institute of Veterinary Medicine of Jiangsu Academy of Agricultural Sciences for their assistance with the experiments. We would like to thank Editage [http://www.editage.cn] for English language editing.

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  1. Yanhua Song and Zhiyu Fan are co-first authors and have contributed equally.

Authors and Affiliations

  1. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing, 210014, China

    Yanhua Song, Zhiyu Fan, Yuanyuan Zuo, Houjun Wei, Bo Hu, Mengmeng Chen, Rulong Qiu, Jiabin Xue & Fang Wang

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  1. Yanhua Song
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  9. Fang Wang
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Correspondence to Fang Wang.

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None of the authors has any financial or personal relationships that could inappropriately influence or bias the content of the paper.

Ethical statement

All animals were handled according to the Standards for Laboratory Animals (GB14925-2001) and the Guideline on the Humane Treatment of Laboratory Animals (MOST 2006a) established by the People’s Republic of China.

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Song, Y., Fan, Z., Zuo, Y. et al. Binding of rabbit hemorrhagic disease virus-like particles to host histo-blood group antigens is blocked by antisera from experimentally vaccinated rabbits. Arch Virol 162, 3425–3430 (2017). https://doi.org/10.1007/s00705-017-3509-8

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  • DOI: https://doi.org/10.1007/s00705-017-3509-8

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