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Complete genome sequence of a novel, virulent Ahjdlikevirus bacteriophage that infects Enterococcus faecium

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Abstract

A novel virulent bacteriophage named vB_EfaP_IME199 that specifically infects Enterococcus faecium was isolated and characterized. Its optimal multiplicity of infection was 0.01, and it had a 30 minute outbreak period. High-throughput sequencing revealed that the phage has a dsDNA genome of 18,838 bp with 22 open reading frames. The genome has very low homology to all other bacteriophage sequences in the GenBank database. Run-off sequencing experiments confirmed that vB_EfaP_IME199 has short inverted terminal repeats. Phylogenetic analysis indicated that vB_EfaP_IME199 can be taxonomically classified as a new member of the genus Ahjdlikevirus of family Podoviridae.

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Acknowledgements

YT, BZ, and LZ conceived and designed the experiments and critically evaluated the manuscript. SX isolated and identified the phage and conducted the biological characterization experiments. XZ was responsible for data and sequence analyses and wrote the manuscript. JW collected clinical bacteria and carried out experiments. QS assembled and analyzed the sequence. GP, SC, ZM, and XA conducted the sequencing experiments. YH helped with sequence analysis. All authors read and approved the final manuscript.

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Author notes

  1. Shaozhen Xing, Xianglilan Zhang and Qiang Sun contributed equally to this work.

Authors and Affiliations

  1. Life Science Institute, Hebei Normal University, Shijiazhuang, 050024, Hebei, China

    Shaozhen Xing & Baohua Zhao

  2. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China

    Xianglilan Zhang, Qiang Sun, Jian Wang, Zhiqiang Mi, Guangqian Pei, Yong Huang, Xiaoping An & Yigang Tong

  3. Central Laboratory, Navy General Hospital, 6 Fucheng Road, Beijing, 100048, China

    Kaifei Fu & Lijun Zhou

Authors
  1. Shaozhen Xing
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  2. Xianglilan Zhang
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  3. Qiang Sun
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  4. Jian Wang
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  9. Kaifei Fu
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  10. Lijun Zhou
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  11. Baohua Zhao
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  12. Yigang Tong
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Corresponding authors

Correspondence to Lijun Zhou, Baohua Zhao or Yigang Tong.

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Conflict of interest

The authors declare that they have no conflict of interest.

Funding

This study was funded by the National Hi-Tech Research and Development (863) Program of China (Nos. 2012AA022003 and 2014AA021402), the China Mega-Project on Infectious Disease Prevention (Nos. 2013ZX10004605, 2011ZX10004001, 2013ZX10004607-004 and 2013ZX10004217-002-003), the State Key Laboratory of Pathogen and BioSecurity Program (No. SKLPBS1113).

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Xing, S., Zhang, X., Sun, Q. et al. Complete genome sequence of a novel, virulent Ahjdlikevirus bacteriophage that infects Enterococcus faecium . Arch Virol 162, 3843–3847 (2017). https://doi.org/10.1007/s00705-017-3503-1

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  • DOI: https://doi.org/10.1007/s00705-017-3503-1

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