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The complete nucleotide sequence and genome organization of a novel betaflexivirus infecting Citrullus lanatus

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Abstract

The complete nucleotide sequence of a novel positive single-stranded (+ss) RNA virus, tentatively named watermelon virus A (WVA), was determined using a combination of three methods: RNA sequencing, small RNA sequencing, and Sanger sequencing. The full genome of WVA is comprised of 8,372 nucleotides (nt), excluding the poly (A) tail, and contains four open reading frames (ORFs). The largest ORF, ORF1 encodes a putative replication-associated polyprotein (RP) with three conserved domains. ORF2 and ORF4 encode a movement protein (MP) and coat protein (CP), respectively. The putative product encoded by ORF3, of an estimated molecular mass of 25 kDa, has no significant similarity with other proteins. Identity and phylogenetic analysis indicate that WVA is a new virus, closely related to members of the family Betaflexiviridae. However, the final taxonomic allocation of WVA within the family is yet to be determined.

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Acknowledgements

This research was supported by the Special Fund for Agro-scientific Research in the Public Interest (201303021), the Knowledge Innovation Program of the Chinese Academy of Agricultural Sciences.

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Authors and Affiliations

  1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

    Min Xin, Peipei Zhang, Wenwen Liu & Xifeng Wang

  2. Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China

    Yingdang Ren

  3. National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing, 400712, China

    Mengji Cao

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  1. Min Xin
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  2. Peipei Zhang
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  3. Wenwen Liu
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  4. Yingdang Ren
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  5. Mengji Cao
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  6. Xifeng Wang
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Corresponding author

Correspondence to Xifeng Wang.

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Xin, M., Zhang, P., Liu, W. et al. The complete nucleotide sequence and genome organization of a novel betaflexivirus infecting Citrullus lanatus . Arch Virol 162, 3239–3242 (2017). https://doi.org/10.1007/s00705-017-3461-7

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  • DOI: https://doi.org/10.1007/s00705-017-3461-7

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