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Genetic characterization of orf virus associated with an outbreak of severe orf in goats at a farm in Lusaka, Zambia (2015)

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Abstract

Orf or contagious ecthyma is a neglected and economically important zoonotic disease caused by a dermatotropic parapoxvirus that commonly affects domestic small ruminants. Although orf is globally distributed, there is a paucity of information on the disease in many African countries. Here, a suspected severe outbreak of orf in goats at a farm in Lusaka was investigated. Orf virus (ORFV) infection was confirmed by PCR amplification of viral DNA (RNA polymerase, B2L and virus interferon-resistance genes) in clinical samples. Some detected genes were sequenced and phylogenetically analyzed. This is the first report on molecular characterization of ORFV in goats in Zambia.

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References

  1. Inoshima Y, Morooka A, Sentsui H (2000) Detection and diagnosis of parapoxvirus by the polymerase chain reaction. J Virol Methods 84:201–208

    Article  CAS  PubMed  Google Scholar 

  2. Kummeneje K, Krogsrud J (1979) Contagious ecthyma (orf) in reindeer (Rangifer tarandus tarandus). Vet Rec 105(3):60–61

    Article  CAS  PubMed  Google Scholar 

  3. Guo J, Rasmussen J, Wünschmann A, de La Concha-Bermejillo A (2004) Genetic characterization of orf viruses isolated from various ruminant species of a zoo. Vet Microbiol 99:81–92

    Article  CAS  PubMed  Google Scholar 

  4. MacLachlan NJ, Dubovi EJ (2011) Poxviridae. In: Fenner’s veterinary virology, 4th edn. Academic, London, pp 151–65

  5. Delhon G, Tulman ER, Afonso CL, Lu Z, de la Concha-Bermejillo A, Lehmkuhl HD, Piccone ME, Kutish GF, Rock DL (2004) Genomes of the parapoxviruses orf virus and bovine popular stomatitis virus. J Virol 78:168–177

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Nandi S, De UK, Chowdhury S (2011) Current status of contagious ecthyma or orf disease in goat and sheep: a global perspective. Small Ruminant Res 96:73–82

    Article  Google Scholar 

  7. Abuelzein EME, Housawi FM (1997) Severe long-lasting contagious ecthyma infection in a goat’s kid. Zentralbl Veterinarmed Reihe B 44:561–564

    CAS  Google Scholar 

  8. Guo J, Zhang Z, Edwards JF, Ermel RW, Taylor C, de La Concha-Bermejillo A (2003) Characterization of a North American orf virus isolated from a goat with persistent, proliferative dermatitis. Virus Res 93(2):169–179

    Article  CAS  PubMed  Google Scholar 

  9. McKeever DJ, Jenkinson DM, Hutchison G, Reid HW (1988) Studies on the pathogenesis of orf virus infection in sheep. J Comp Pathol 99:317–328

    Article  CAS  PubMed  Google Scholar 

  10. Rajkomar V, Hannah M, Coulson IH, Owen CM (2016) A case of human to human transmission of orf between mother and child. Clin Exp Dermatol 41(1):60–63

    Article  CAS  PubMed  Google Scholar 

  11. Uzel M, Sasmaz S, Bakaris S, Cetinus E, Bilgic E, Karaoguz A, Ozkul A, Arican O (2005) A viral infection of the hand commonly seen after the feast of sacrifice: human orf (orf of the hand). Epidemiol Infect 133:653–657

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Centers for Disease Control and Prevention (CDC) (2012) Human Orf virus infection from household exposures-United States, 2009–2011. MMWR Morb Mortal Wkly Rep 61(14):245

    Google Scholar 

  13. Gelaye E, Achenbach JE, Jenberie S, Ayelet G, Belay A, Yami M, Loitsch A, Grabherr R, Diallo A, Lamien CE (2016) Molecular characterization of orf virus from sheep and goats in Ethiopia, 2008–2013. Virol J 13:34

    Article  PubMed  PubMed Central  Google Scholar 

  14. Maganga GD, Relmy A, Bakkali-Kassimi L, Ngoubangoye B, Tsoumbou T, Bouchier C, N’Dilimabaka N, Leroy EM, Zientara S, Berthet N (2016) Molecular characterization of Orf virus in goats in Gabon, Central Africa. Virol J 13(1):1

    Article  CAS  Google Scholar 

  15. Scagliarini A, Piovesana S, Turrini F, Savini F, Sithole F, McCrindle CM (2012) Orf in South Africa: endemic but neglected. Onderstepoort J Vet Res 79(1):1–8. doi:10.4102/ojvr.v79i1.499

    Article  Google Scholar 

  16. Selim A, Elhaig M, Höche J, Gaede W (2016) Molecular detection and analysis of Sheeppox and Orf viruses isolated from sheep from Qalubia, Egypt. Berl Munch Tierarztl Wochenschr 129(7-8):310–317

    PubMed  Google Scholar 

  17. Moallin ASM, Zessin KH (1988) Outbreak of camel contagious ecthyma in Central Somalia. Trop Anim Health Prod 20:185–186

    Article  CAS  PubMed  Google Scholar 

  18. Torfason EG, Gunadottir S (2002) Polymerase chain reaction for laboratory diagnosis of orf virus infections. J Clin Virol 24:79–84

    Article  CAS  PubMed  Google Scholar 

  19. Ndashe K, Simulundu E, Hang’ombe BM, Moonga L, Ogawa H, Takada A, Mweene AS (2016) Molecular characterization of infectious bursal disease viruses detected in vaccinated commercial broiler flocks in Lusaka, Zambia. Arch Virol 161(3):513–519

    Article  CAS  PubMed  Google Scholar 

  20. Chan KW, Yang CH, Lin JW, Wang HC, Lin FY, Kuo ST, Wong ML, Hsu WL (2009) Phylogenetic analysis of parapoxviruses and the C-terminal heterogeneity of viral ATPase proteins. Gene 432(1):44–53

    Article  CAS  PubMed  Google Scholar 

  21. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Tamura K (1992) Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G + C-content biases. Mol Biol Evol 9:678–687

    CAS  PubMed  Google Scholar 

  23. Obi TU, Gibbs EP (1978) Orf in sheep and goats in Nigeria. Trop Anim Health Prod 10:233–235

    Article  CAS  PubMed  Google Scholar 

  24. Rogers M, Bale P, De Silva LM, Glasson MJ, Collins E (1989) Giant parapox infection in a two year old child. Australas J Dermatol 30(2):87–91

    Article  CAS  PubMed  Google Scholar 

  25. Lederman ER, Green GM, DeGroot HE, Dahl P, Goldman E, Greer PW, Li Y, Zhao H, Paddock CD, Damon IK (2007) Progressive Orf virus infection in a patient with lymphoma: successful treatment using imiquimod. Clin Infect Dis 44:e100–3

    Article  PubMed  Google Scholar 

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Acknowledgements

The researchers would like to thank the University of Zambia, School of Veterinary Medicine for providing support staff, laboratory and clinical space. This work was supported by the Japan Initiative for Global Research Network on Infectious Diseases (J-GRID) and the Japan Agency for Medical Research and Development (AMED)/Japan International Cooperation Agency (JICA) within the framework of the Science and Technology Research Partnership for Sustainable Development (SATREPS).

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Author notes

  1. Nandi Mtine and Thoko F. Kapalamula contributed equally to this work.

Authors and Affiliations

  1. Department of Disease Control, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia

    Edgar Simulundu, Thoko F. Kapalamula, Ayato Takada & Aaron S. Mweene

  2. Department of Biomedical Sciences, School of Health Sciences, University of Zambia, PO Box 50110, Lusaka, Zambia

    Nandi Mtine, Geoffrey Kwenda & Chrispin Chisanga

  3. Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan

    Masahiro Kajihara & Ayato Takada

  4. Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia

    Yongjin Qiu

  5. Department of Clinical Studies, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia

    James Ngoma, Victor Zulu & Isaac K. Phiri

  6. Global Institution for Collaborative Research and Education, Hokkaido University, Sapporo, Japan

    Ayato Takada

Authors
  1. Edgar Simulundu
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  2. Nandi Mtine
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  3. Thoko F. Kapalamula
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  4. Masahiro Kajihara
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  5. Yongjin Qiu
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  6. James Ngoma
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  7. Victor Zulu
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  8. Geoffrey Kwenda
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  9. Chrispin Chisanga
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  10. Isaac K. Phiri
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  11. Ayato Takada
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  12. Aaron S. Mweene
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Corresponding author

Correspondence to Edgar Simulundu.

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Simulundu, E., Mtine, N., Kapalamula, T.F. et al. Genetic characterization of orf virus associated with an outbreak of severe orf in goats at a farm in Lusaka, Zambia (2015). Arch Virol 162, 2363–2367 (2017). https://doi.org/10.1007/s00705-017-3352-y

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  • DOI: https://doi.org/10.1007/s00705-017-3352-y

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