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Characterization and genome comparisons of three Achromobacter phages of the family Siphoviridae

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Abstract

In this study, we present the characterization and genomic data of three Achromobacter phages belonging to the family Siphoviridae. Phages 83-24, JWX and JWF were isolated from sewage samples in Paris and Braunschweig, respectively, and infect Achromobacter xylosoxidans, an emerging nosocomial pathogen in cystic fibrosis patients. Analysis of morphology and growth parameters revealed that phages 83-24 and JWX have similar properties, both have nearly the same head and tail measurements, and both have a burst size between 85 and 100 pfu/cell. In regard to morphological properties, JWF had a much longer and more flexible tail compared to other phages. The linear double-stranded DNAs of all three phages are terminally redundant and not circularly permutated. The complete nucleotide sequences consist of 81,541 bp for JWF, 49,714 bp for JWX and 48,216 bp for 83-24. Analysis of the genome sequences showed again that phages JWX and 83-24 are quite similar. Comparison to the GenBank database via BLASTN revealed partial similarities to Roseobacter phage RDJL phi1 and Burkholderia phage BcepGomr. In contrast, BLASTN analysis of the genome sequence of phage JWF revealed only few similarities to non-annotated prophage regions in different strains of Burkholderia and Mesorhizobium.

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Acknowledgements

We sincerely thank Simone Severitt, Nicole Heyer and Anja Meier for technical assistance. We further thank Professor Ed Moore (CCUG Culture Collection, University of Gothenburg, Sweden) and Dr. Danielle Janssens (BCCM/LMG, Laboratory for Microbiology, University of Gent, Belgium) for providing us with strains of Achromobacter xylosoxidans, and Professor Sylvain Moineau (Félix d`Hérelle Reference Center for Bacterial Viruses, Université Laval, Canada) for phage 83-24.

Author contributions

BD conceived and designed the experiments and wrote the paper, BB and CS performed sequencing and genome analysis, MR performed morphological analysis via TEM microscopy, MN analyzed the peptide mass fingerprinting data, JW conceived and designed the experiments, analyzed data and wrote the paper.

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Authors and Affiliations

  1. Department of Microbiology/Genetechnology, University of Bielefeld, 33615, Bielefeld, Germany

    Brigitte Dreiseikelmann

  2. Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, 38124, Brunswick, Germany

    Boyke Bunk, Cathrin Spröer & Johannes Wittmann

  3. Central Facility for Microscopy, Helmholtz Centre for Infection Research, 38124, Brunswick, Germany

    Manfred Rohde

  4. Protein Analytics Platform, Helmholtz Centre for Infection Research, 38124, Brunswick, Germany

    Manfred Nimtz

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  1. Brigitte Dreiseikelmann
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  2. Boyke Bunk
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Correspondence to Johannes Wittmann.

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Dreiseikelmann, B., Bunk, B., Spröer, C. et al. Characterization and genome comparisons of three Achromobacter phages of the family Siphoviridae . Arch Virol 162, 2191–2201 (2017). https://doi.org/10.1007/s00705-017-3347-8

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