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Molecular characterization of a new begomovirus infecting Mirabilis jalapa in northern India

Archives of Virology volume 162, pages 2163–2167 (2017)Cite this article

Abstract

Begomoviruses are whitefly-transmitted single-stranded DNA viruses that are responsible for considerable economic losses. A begomovirus, alphasatellite and betasatellite were characterized in a Mirabilis jalapa plant exhibiting severe leaf curling and mottling symptoms. The complete viral genome shared highest sequence identity of 87% with pedilanthus leaf curl virus (AM712436), reported from Pakistan. Additionally, the viral genome was 84% identical to that of chilli leaf curl India virus (KX951415) and 83% identical to that of tobacco curly shoot virus (GU1999584), which were previously reported to infect M. jalapa in India and China, respectively. Based on the ICTV criterion for begomovirus species demarcation (≥91% sequence identity for the complete genome), the virus represents a new species, for which we propose the name Mirabilis leaf curl virus. The alphasatellite and betasatellite sequences were similar to the corresponding sequences of ageratum yellow vein India alphasatellite (KU852743; 99% identity) and tomato leaf curl Patna betasatellite (HQ180394; 86% identity) sequences, respectively. This report describes a new begomovirus-satellite disease complex in M. jalapa.

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Acknowledgements

We are very thankful to the Director of CSIR-IHBT, Palampur, for providing necessary research facilities to carry out the work. We also gratefully acknowledge Dr. Robert W. Briddon, National Institute of Biotechnology and Genetic Engineering, Faisalabad, Pakistan, for valuable discussions regarding betasatellites and satellite conserved regions (SCRs). The Fellowships to AK and PR were provided by the University Grants Commission (UGC) and Council of Scientific and Industrial Research (CSIR), respectively, and are duly acknowledged. We also gratefully acknowledge Dr. Rachael Oakenfull and Dr. Thomas Hartley, University of York (UK), for manuscript editing, and the Academy of Scientific and Innovative Research (AcSIR), New Delhi. CSIR is acknowledged for financial support. This is CSIR-IHBT publication number 3849.

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  1. Academy of Scientific and Innovative Research (AcSIR), CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India

    Aditya Kulshreshtha, Poonam Roshan & Vipin Hallan

  2. CSIR-IHBT, Palampur, Himachal Pradesh, 176061, India

    Aditya Kulshreshtha, Poonam Roshan, Dolly Sharma & Vipin Hallan

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  1. Aditya Kulshreshtha
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  2. Poonam Roshan
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Correspondence to Vipin Hallan.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Council of Scientific and Industrial Research (fellowship to PR), University Grants Commission (fellowship to AK).

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Kulshreshtha, A., Roshan, P., Sharma, D. et al. Molecular characterization of a new begomovirus infecting Mirabilis jalapa in northern India. Arch Virol 162, 2163–2167 (2017). https://doi.org/10.1007/s00705-017-3330-4

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  • DOI: https://doi.org/10.1007/s00705-017-3330-4

Keywords

  • Bipartite Begomoviruses
  • Monopartite Begomoviruses
  • Coat Protein Gene Sequence
  • Complete Viral Genome
  • Tobacco Curly Shoot Virus