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Cloning, sequencing and characterization of the genome of a recombinant norovirus of the rare genotype GII.P7/GII.6 in China

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Abstract

The genome sequence of a rare recombinant norovirus (NoV) genotype obtained from clinical samples in China was determined using one-step reverse transcription PCR. It was identified as the GII.P7/GII.6 genotype using both phylogenetic and SimPlot analyses. A high degree of variability was observed in the P2 subdomain, especially in the B-loop structure. The recombination breakpoints of all available GII.P7/GII.6 strains were mapped to two different positions within the RdRp region, both of which were at least 40 nt upstream of the overlap of ORF1 and 2. The GII.P7/GII.6 genotype appears to have been circulating in Asia for at least 10 years.

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Acknowledgements

This work was supported partially by the National Natural Science Foundation of China (41376135, 31570112 and 31601570) and the Special Fund for the Development of Science and Technology of Shanghai Ocean University (2015).

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Author notes

  1. H. Cai and Y. Yu contributed equally to this paper.

Authors and Affiliations

  1. College of Food Science and Technology, Shanghai Ocean University, Shanghai, China

    Hui Cai, Yongxin Yu, Yingjie Pan, Shuling Yan & Yongjie Wang

  2. Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai, China

    Yongxin Yu, Yingjie Pan & Yongjie Wang

  3. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China

    Miao Jin

  4. Institute of Biochemistry and Molecular Cell Biology, University of Göttingen, Göttingen, Germany

    Shuling Yan

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  1. Hui Cai
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  2. Yongxin Yu
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  3. Miao Jin
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  4. Yingjie Pan
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  5. Shuling Yan
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Correspondence to Yongjie Wang.

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This article does not contain any studies with animals performed by any of the authors.

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Cai, H., Yu, Y., Jin, M. et al. Cloning, sequencing and characterization of the genome of a recombinant norovirus of the rare genotype GII.P7/GII.6 in China. Arch Virol 162, 2053–2059 (2017). https://doi.org/10.1007/s00705-017-3325-1

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  • DOI: https://doi.org/10.1007/s00705-017-3325-1

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