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Characterization of a novel strain of Botryosphaeria dothidea chrysovirus 1 from the apple white rot pathogen Botryosphaeria dothidea

Abstract

A novel strain of Botryosphaeria dothidea chrysovirus 1 was identified. It encodes a shortened RNA-dependent RNA polymerase and an elongated coat protein, and it might cause hypovirulence of the host fungal strain.

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Correspondence to Li-Yun Guo.

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Conflict of interest

The authors declare that they have no conflict of interest. This study did not include experiments with human participants or animals performed by any of the authors.

Funding

This work was supported by the Earmarked Fund for Modern Agro-Industry Technology Research System (CARS-28).

Electronic supplementary material

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705_2017_3320_MOESM1_ESM.pdf

Figure S1. Characterization of BdCV1-G1 RNA components, virion particles and structural proteins. (a) Agarose gel electrophoresis (1.0%) profile of dsRNA preparations extracted from mycelia of ZY7 and G1 after digestion with DNase I and S1 nuclease. Marker, Takara Wide Range DNA Marker (500-12,000 bp). (b) Agarose gel (1.0%) electrophoresis profile of BdCV1-G1 genomic dsRNA segments. dsRNAs were isolated from purified BdCV1-G1 virions (V-BdCV1-G1) and mycelia (M-BdCV1-G1). Marker, Takara Wide Range DNA Marker (500-12,000 bp). (c) Transmission electron micrograph of BdCV1-G1 virus particles from fungal strain G1. The particles were stained negatively with 2% uranyl acetate. (d) SDS-PAGE analysis of proteins purified from virion preparations from G1 mycelia (P-G1) and strain ZY7 (P-ZY7). Marker, protein molecular mass marker. * indicates the structural proteins of the virions. (e) Northern blot analysis of the BdCV1-G1 genomic dsRNAs. Probe sequences are listed in Table S1 (PDF 2001 kb)

705_2017_3320_MOESM2_ESM.pdf

Figure S2. Multiple sequence alignments of the 5’- and 3’- terminal regions of the four dsRNAs of the BdCV1-G1 to BdCV1 consensus sequence (a) and phylogenetic analyses of RdRp of BdCV1-G1 and representative members of the families Chrysoviridae and Partitiviridae (b). Shading: black, 100% nucleotide sequence identity; gray, 60–80% nucleotide sequence identity. The BdCV1-conserved sequence in the 5’- and 3’- terminal regions is shown in the last line of the alignments, and star dots show identical nucleotides. The unrooted phylogenetic tree was constructed based on the neighbor-joining method using the program MEGA 5.0 with 1000 bootstrap replicates. The scale bar corresponded to the genetic distance (PDF 1107 kb)

705_2017_3320_MOESM3_ESM.pdf

Figure S3. Pairwise alignment of the ORF1 deduced amino acid sequences of BdCV1-G1 and BdCV1. Black shading indicates identical amino acids (PDF 392 kb)

705_2017_3320_MOESM4_ESM.pdf

Figure S4. Pairwise alignment of the ORF2 deduced amino acid sequences of BdCV1-G1 and BdCV1. Black shading indicates identical amino acids (PDF 367 kb)

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Ding, Z., Zhou, T. & Guo, LY. Characterization of a novel strain of Botryosphaeria dothidea chrysovirus 1 from the apple white rot pathogen Botryosphaeria dothidea . Arch Virol 162, 2097–2102 (2017). https://doi.org/10.1007/s00705-017-3320-6

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  • DOI: https://doi.org/10.1007/s00705-017-3320-6

Keywords

  • Terminal Sequence
  • dsRNA Virus
  • Chestnut Blight
  • Apple Shoot
  • Hypovirulent Strain