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Immune response of gilts to single and double infection with porcine epidemic diarrhea virus

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Abstract

Immune response of gilts following single and double infection with porcine epidemic diarrhea virus (PEDV) at gilt acclimatization and prepartum were investigated. One hundred PEDV-naïve gilts were divided into two groups: negative (Neg) and feedback (FB) groups. Antibody responses in serum, colostrum, and milk samples were measured by IgG/IgA ELISA and virus neutralization assay (VN). Fecal shedding was investigated using RT-PCR. In summary, a single infection at gilt acclimatization resulted in slightly increased serum antibody titers as determined by VN assay and IgG ELISA, but not by IgA ELISA. Viral RNA was detected in fecal samples up to 6 days post-exposure. A double infection at prepartum resulted in significantly increased IgA and VN titers in milk samples compared to the single-infection group. No fecal shedding was detected following the double infection.

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Acknowledgements

The authors are grateful to Agricultural Research Development Agency (public organization), National Research Council of Thailand, and the Thailand Research Fund. Partial funding was provided by the Special Task Force for Activating Research (STAR), Swine Viral Evolution and Vaccine Research (SVEVR), Chulalongkorn University. The authors are thankful to Dr. Matthew Wegner, faculty of Veterinary Science, for manuscript editing.

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Authors and Affiliations

  1. Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Henry Dunant Road, Pathumwan, Bangkok, 10330, Thailand

    Anchalee Srijangwad, Christopher James Stott, Gun Temeeyasen & Dachrit Nilubol

  2. Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand

    Raweewan Senasuthum, Wanchai Chongcharoen & Angkana Tantituvanont

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  1. Anchalee Srijangwad
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  2. Christopher James Stott
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  3. Gun Temeeyasen
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  4. Raweewan Senasuthum
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  6. Angkana Tantituvanont
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  7. Dachrit Nilubol
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Correspondence to Dachrit Nilubol.

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The study was funded by the Thailand Research Fund (grant number PHD57I0026) and Government Budget Year 2015.

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The authors declare that they have no conflicts of interest related to this work.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Srijangwad, A., Stott, C.J., Temeeyasen, G. et al. Immune response of gilts to single and double infection with porcine epidemic diarrhea virus. Arch Virol 162, 2029–2034 (2017). https://doi.org/10.1007/s00705-017-3307-3

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  • DOI: https://doi.org/10.1007/s00705-017-3307-3

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