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Next generation sequencing and molecular analysis of artichoke Italian latent virus

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Abstract

Next-generation sequencing (NGS) allowed the assembly of the complete RNA-1 and RNA-2 sequences of a grapevine isolate of artichoke Italian latent virus (AILV). RNA-1 and RNA-2 are 7,338 and 4,630 nucleotides in length excluding the 3’ terminal poly(A) tail, and encode two putative polyproteins of 255.8 kDa (p1) and 149.6 kDa (p2), respectively. All conserved motifs and predicted cleavage sites, typical for nepovirus polyproteins, were found in p1 and p2. AILV p1 and p2 share high amino acid identity with their homologues in beet ringspot virus (p1, 81% and p2, 71%), tomato black ring virus (p1, 79% and p2, 63%), grapevine Anatolian ringspot virus (p1, 65% and p2, 63%), and grapevine chrome mosaic virus (p1, 60% and p2, 54%), and to a lesser extent with other grapevine nepoviruses of subgroup A and C. Phylogenetic and sequence analyses, all confirmed the strict relationship of AILV with members classified in subgroup B of genus Nepovirus.

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Authors and Affiliations

  1. Istituto Agronomico Mediterraneo di Bari, Via Ceglie 9, Valenzano, 70010, Bari, Italy

    Toufic Elbeaino, Imen Belghacem & Michele Digiaro

  2. Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università degli Studi di Bari, Via G. Amendola, 165/A, 70126, Bari, Italy

    Tiziana Mascia & Donato Gallitelli

  3. Unità Operativa di Supporto di Bari, Istituto del CNR per la Protezione Sostenibile della Piante, Via G. Amendola 165/A, 70126, Bari, Italy

    Tiziana Mascia & Donato Gallitelli

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  1. Toufic Elbeaino
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  2. Imen Belghacem
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  3. Tiziana Mascia
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  4. Donato Gallitelli
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  5. Michele Digiaro
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Correspondence to Toufic Elbeaino.

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Elbeaino, T., Belghacem, I., Mascia, T. et al. Next generation sequencing and molecular analysis of artichoke Italian latent virus. Arch Virol 162, 1805–1809 (2017). https://doi.org/10.1007/s00705-017-3290-8

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