Abstract
Pepper chlorotic spot virus (PCSV), newly found in Taiwan, was identified as a new tospovirus based on the molecular characterization of its S RNA. In this study, the complete M and L RNA sequences of PCSV were determined. The M RNA has 4795 nucleotides (nts), encoding the NSm protein of 311 aa (34.5 kDa) in the viral (v) strand and the glycoprotein precursor (Gn/Gc) of 1122 aa (127.6 kDa) in the viral complementary (vc) strand. The L RNA has 8859 nts, encoding the RNA-dependent RNA polymerase (RdRp) of 2873 aa (330.8 kDa) in the vc strand. Analyses of the NSm, Gn/Gc and RdRp of PCSV revealed that PCSV is phylogenetically clustered within the watermelon silver mottle virus-related clade. Based on the whole genome sequence, PCSV is closely related to Tomato necrotic ringspot virus and should be classified as a new tospovirus species.
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We are grateful to Dr. Chung-Jan Chang, Professor Emeritus of the University of Georgia and Dr. Kuang-Ren Chung for their critical review of the manuscript.
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This study was partially supported by a grant (NSC 102-2628-B-005-005-MY3) from the National Science Council of Taiwan and a grant (MOST 105-2313-B-005-021-MY3) from the Ministry of Science and Technology, Executive Yuan, Taiwan.
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K.-S. Huang and C.-H. Tai contributed equally to this study.
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Huang, KS., Tai, CH., Cheng, YH. et al. Complete nucleotide sequences of M and L RNAs from a new pepper-infecting tospovirus, Pepper chlorotic spot virus. Arch Virol 162, 2109–2113 (2017). https://doi.org/10.1007/s00705-017-3260-1
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DOI: https://doi.org/10.1007/s00705-017-3260-1