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DDX50 inhibits the replication of dengue virus 2 by upregulating IFN-β production

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Abstract

Dengue virus (DENV) infects approximately 390 million people per year, and each of the four DENV serotypes (DENV-1, DENV-2, DENV-3, and DENV-4) is capable of causing infection. At present, there is no antiviral drug available for the treatment of DENV. Several DExD/H-box helicases have been shown to be involved in the antiviral immune response or viral replication. In the present study, we investigated the role of DDX50 in DENV-2 RNA replication. Our data showed that the level of DENV-2 RNA increased in DDX50 knockdown cells during an early stage of viral infection and decreased in DDX50-overexpressing cells. DDX50, in conjunction with RIG-I and MDA5, upregulated the production of IFN-β in infected cells through an additive effect on the IFN-β promoter. Furthermore, transcription of several IFN-stimulated genes was increased in DDX50-overexpressing cells infected with DENV-2. These results provide evidence that DDX50 negatively regulates DENV-2 replication during the early stages of infection by inducing IFN-β production.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (no. 31370193).

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    Authors and Affiliations

    1. The Department of Microbiology, School of Preclinical Medicine, The Fourth Military Medical University, No.169, Changle West Road, Xian, 710032, Shaanxi, China

      Peijun Han, Wei Ye, Xin Lv, Hongwei Ma, Daihui Weng, Yangchao Dong, Linfeng Cheng, Hesong Chen, Liang Zhang, Zhikai Xu, Yingfeng Lei & Fanglin Zhang

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    1. Peijun Han
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    Correspondence to Yingfeng Lei or Fanglin Zhang.

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    P. Han, W. Ye, X. Lv contributed equally to this article.

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    Han, P., Ye, W., Lv, X. et al. DDX50 inhibits the replication of dengue virus 2 by upregulating IFN-β production. Arch Virol 162, 1487–1494 (2017). https://doi.org/10.1007/s00705-017-3250-3

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    • DOI: https://doi.org/10.1007/s00705-017-3250-3

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