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Archives of Virology

, Volume 162, Issue 5, pp 1373–1379 | Cite as

Complete nucleotide sequence of clematis chlorotic mottle virus, a new member of the family Tombusviridae

  • Margaret McLaughlin
  • Ben Lockhart
  • Ramon Jordan
  • Geoff Denton
  • Dimitre MollovEmail author
Brief Report

Abstract

Clematis chlorotic mottle virus (ClCMV) is a previously undescribed virus associated with symptoms of yellow mottling and veining, chlorotic ring spots, line pattern mosaics, and flower distortion and discoloration on ornamental Clematis. The ClCMV genome is 3,880 nt in length with five open reading frames (ORFs) encoding a 27-kDa protein (ORF 1), an 87-kDa replicase protein (ORF 2), two centrally located movement proteins (ORF 3 and 4), and a 37-kDa capsid protein (ORF 5). Based on morphological, genomic, and phylogenetic analysis, ClCMV is predicted to be a member of the genus Pelarspovirus in the family Tombusviridae.

Keywords

Capsid Protein Movement Protein Tomato Spotted Wilt Virus Yellow Mottling Tobacco Streak Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Compliance with ethical standards

All authors declare they have no conflict of interest. The research reported in this manuscript did not involve experimentation with human or animal subjects. Plant samples were moved within the U.S.A. under USDA APHIS permit P526P-13-03719 and from the U.K. under permit P526P-14-00688.

Supplementary material

705_2017_3236_MOESM1_ESM.docx (13 kb)
Supplementary material 1 (DOCX 12 kb)
705_2017_3236_MOESM2_ESM.tif (31 kb)
Supplementary Figure S1 An internal replication element (IRE) indentified by Mfold RNA secondary structure prediction analysis within the RdRp of the genome of ClCMV is similar to those found in other members of the family Tombusviridae, including viruses of the genus Pelarspovirus. This IRE contains one distinct conserved motif (5’-CAU UCC-3’/5’-GGC AGG-3’) within the Tombusvirdae IRE motif 2, which has been identified in other members of the genus Pelarspovirus and is in ClCMV at nt 1020 and nt 1069. The nucleotides in the internal loop motifs that are conserved within the genus Pelarspovirus are shown in red, and nucleotides corresponding to the CC mismatch in tombusviruses are shown in green. (TIFF 30 kb)

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Copyright information

© Springer-Verlag Wien (Outside the USA) 2017

Authors and Affiliations

  1. 1.USDA ARS, National Germplasm Resources LaboratoryBeltsvilleUSA
  2. 2.Department of Plant PathologyUniversity of MinnesotaSt. PaulUSA
  3. 3.USDA ARS, U.S. National Arboretum, Floral and Nursery Plant Research UnitBeltsvilleUSA
  4. 4.Royal Horticultural SocietySurreyUK

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