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Genetic properties and pathogenicity of a novel reassortant H10N5 influenza virus from wild birds

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Abstract

In this study, we analyzed the genome of a H10N5 influenza virus from wild birds. This virus was identified as a novel reassortant virus with internal genes from multiple subtypes and of distinct origins. After sequential passage in mice, mouse-adapted viruses bearing mutations PB2-E627K and HA-G218E were generated. These viruses caused dramatic body weight loss and death, and also replicated in mouse brain, suggesting that the pathogenicity of low pathogenic H10N5 in chickens can be enhanced after passage in mammals. Our data imply that H10N5 viruses might be a potential risk to human health therefore it is important to undertake continued surveillance and biosecurity evaluation of these viruses.

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    Authors and Affiliations

    1. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou, 730046, China

      Yane Jia, Jiayun Yang, Zhengxiang Wang, Yingying Du, Liang Wang, Fengfeng Guo, Maijuan Yang, Shufang Han & Qiyun Zhu

    2. Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China

      Jie Cui

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    1. Yane Jia
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    Correspondence to Qiyun Zhu.

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    Funding

    This work was supported by funding from the National Key R&D program (2016YFD0500207, 2016YFD0500201) and the National Natural Science Foundation of China (81571998). Jie Cui is supported by the CAS Pioneer Hundred Talents Program.

    Conflict of interest

    All authors have read the manuscript and the authors declare that they have no conflicts of interest.

    Ethical approval

    This article does not contain any studies with human participants. The animal studies were conducted within the animal biosafety level 3 (ABSL3) facilities in the Lanzhou Veterinary Research Institute (LVRI) of Chinese Academy of Agricultural Sciences (CAAS). The protocols for animal studies were approved by the Committee on the Ethics of Animal Experiments of LVRI.

    Additional information

    Y. Jia and J. Yang contributed equally to this work.

    Electronic supplementary material

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    705_2017_3234_MOESM1_ESM.pdf

    Figure S1: Phylogenetic analysis of A/wild bird/Hunan/270/2014 HA and NA genes. The evolutionary history was inferred using HA (A) and NA (B) gene alignments. The tree is drawn to scale with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed and are in units of the number of base substitutions per site. The rate variation among sites was modeled by gamma distribution. The coding regions of HA and NA genes were used (n = 64 sequences) for phylogenetic analysis. Bootstrap values lower than 70% are not shown. The H10N5 isolated in this study is highlighted in both HA and NA trees. (PDF 639 kb)

    Supplementary material 2 (PDF 63 kb)

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    Jia, Y., Yang, J., Wang, Z. et al. Genetic properties and pathogenicity of a novel reassortant H10N5 influenza virus from wild birds. Arch Virol 162, 1349–1353 (2017). https://doi.org/10.1007/s00705-017-3234-3

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    • DOI: https://doi.org/10.1007/s00705-017-3234-3

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